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PMID: 8953772 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase.

The Plant cell ·Vol. 8 ·No. 11 ·1996-11-00 ·Pages 2079-91

Ueda T, Matsuda N, Anai T, Tsukaya H, Uchimiya H, Nakano A

Abstract

The Arabidopsis Ara proteins belong to the Rab/Ypt family of small GTPases, which are implicated in intracellular vesicular traffic. To understand their specific roles in the cell, it is imperative to identify molecules that regulate the GTPase cycle. Such molecules have been found and characterized in animals and yeasts but not in plants. Using a yeast system, we developed a novel method of functional screening to detect interactions between foreign genes and identified this Rab regulator in plants. We found that the expression of the ARA4 gene in yeast ypt mutants causes exaggeration of the mutant phenotype. By introducing an Arabidopsis cDNA library into the ypt1 mutant, we isolated a clone whose coexpression overcame the deleterious effect of ARA4. This gene encodes an Arabidopsis homolog of the Rab GDP dissociation inhibitor (GDI) and was named AtGDI1. The expression of AtGDI1 complemented the yeast sec19-1 (gdi1) mutation. AtGDI1 is expressed almost ubiquitously in Arabidopsis tissues. The method described here indicates the physiological interaction of two plant molecules, Ara4 and GDI, in yeast and should be applicable to other foreign genes.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Arabidopsis Proteins Base Sequence GTP Phosphohydrolases/metabolism GTP-Binding Proteins/antagonists & inhibitors,genetics Genes, Suppressor Guanine Nucleotide Dissociation Inhibitors Microscopy, Electron Molecular Sequence Data Sequence Alignment rab GTP-Binding Proteins rho-Specific Guanine Nucleotide Dissociation Inhibitors
Chemicals
ARA4 protein, Arabidopsis Arabidopsis Proteins Guanine Nucleotide Dissociation Inhibitors rho-Specific Guanine Nucleotide Dissociation Inhibitors GTP Phosphohydrolases GTP-Binding Proteins rab GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ueda T
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Matsuda N
Anai T
Tsukaya H
Uchimiya H
Nakano A
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.8.11.2079
Published
1996-11-00
Pages
2079-91
Language
English
Region
England
NLM ID
9208688
PMCID
PMC161336
Subset
IM
Databases
GENBANK
D83531
External Links
PubMed source DOI: 10.1105/tpc.8.11.2079 PMC Full Text View journal details
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