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PMID: 8917519 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3' end.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 23 ·1996-11-12 ·Pages 12914-9

Tschochner H

Abstract

A novel RNase activity was identified in a yeast RNA polymerase I (pol I) in vitro transcription system. Transcript cleavage occurred at the 3' end and was dependent on the presence of ternary pol I/DNA/RNA complexes and an additional protein factor not identical to transcription factor IIS (TFIIS). Transcript cleavage was observed both on arrested complexes at the linearized ends of the transcribed DNA and on intrinsic blocks of the DNA template. Shortened transcripts that remained associated within the ternary complexes were capable of resuming RNA chain elongation. Possible functions of the nuclease for transcript elongation or termination are discussed.

MeSH Terms
Base Sequence DNA, Fungal/metabolism Mitochondria/enzymology Molecular Sequence Data Oligodeoxyribonucleotides Protein Binding RNA Polymerase I/metabolism RNA, Fungal/metabolism Ribonucleases/metabolism Saccharomyces cerevisiae/genetics,metabolism Templates, Genetic Transcription Factors/metabolism Transcription Factors, General Transcription, Genetic Transcriptional Elongation Factors
Chemicals
DNA, Fungal Oligodeoxyribonucleotides RNA, Fungal Transcription Factors Transcription Factors, General Transcriptional Elongation Factors transcription factor S-II RNA Polymerase I Ribonucleases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Tschochner H
Institut für Biochemie I, Universität Heidelberg, Germany. IM4@ix.urz.uni-heidelberg.de
References (36)
36 references, click to expand
  1. Association of RNase H activity with yeast RNA polymerase A.
    Nature. 1976 Jun 3;261(5559):431-3 PMID: 778628
  2. RNA polymerase II ternary complexes may become arrested after transcribing to within 10 bases of the end of linear templates.
    J Biol Chem. 1995 Feb 3;270(5):2290-7 PMID: 7836462
  3. 3'-end formation of mouse pre-rRNA involves both transcription termination and a specific processing reaction.
    Genes Dev. 1989 Feb;3(2):224-31 PMID: 2714650
  4. Use of yeast nuclear DNA sequences to define the mitochondrial RNA polymerase promoter in vitro.
    Mol Cell Biol. 1989 Aug;9(8):3193-202 PMID: 2677667
  5. Transcription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Aug;9(8):3464-72 PMID: 2677675
  6. Specific interaction of the murine transcription termination factor TTF I with class-I RNA polymerases.
    Nature. 1990 Apr 5;344(6266):559-62 PMID: 2181320
  7. Specific transcription of Saccharomyces cerevisiae 35 S rDNA by RNA polymerase I in vitro.
    J Biol Chem. 1990 May 5;265(13):7596-603 PMID: 2185253
  8. RNA chain elongation by Escherichia coli RNA polymerase. Factors affecting the stability of elongating ternary complexes.
    J Mol Biol. 1990 May 5;213(1):79-108 PMID: 1692594
  9. Accurately initiated, enhancer-dependent transcription by RNA polymerase I in yeast extracts.
    J Biol Chem. 1990 Oct 25;265(30):18091-4 PMID: 2211685
  10. Specific initiation by RNA polymerase I in a whole-cell extract from yeast.
    Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):1004-8 PMID: 1992452
  11. Spontaneous cleavage of RNA in ternary complexes of Escherichia coli RNA polymerase and its significance for the mechanism of transcription.
    Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7983-7 PMID: 1716768
  12. Elongation factor-dependent transcript shortening by template-engaged RNA polymerase II.
    J Biol Chem. 1992 Feb 25;267(6):3795-800 PMID: 1371280
  13. Interaction of BiP with newly synthesized immunoglobulin light chain molecules: cycles of sequential binding and release.
    EMBO J. 1992 Apr;11(4):1573-81 PMID: 1563355
  14. The RNA polymerase II ternary complex cleaves the nascent transcript in a 3'----5' direction in the presence of elongation factor SII.
    Genes Dev. 1992 Jul;6(7):1342-56 PMID: 1378419
  15. The RNA polymerase II elongation complex. Factor-dependent transcription elongation involves nascent RNA cleavage.
    J Biol Chem. 1992 Aug 5;267(22):15516-22 PMID: 1379232
  16. Simple derivation of TFIID-dependent RNA polymerase II transcription systems from Schizosaccharomyces pombe and other organisms, and factors required for transcriptional activation.
    Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7659-63 PMID: 1502179
  17. GreA protein: a transcription elongation factor from Escherichia coli.
    Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8899-902 PMID: 1384037
  18. Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae.
    J Biol Chem. 1992 Nov 15;267(32):23376-82 PMID: 1331084
  19. Nascent RNA cleavage by purified ternary complexes of vaccinia RNA polymerase.
    J Biol Chem. 1993 Jan 25;268(3):2166-73 PMID: 7678416
  20. Identification of a 3'-->5' exonuclease activity associated with human RNA polymerase II.
    Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):843-7 PMID: 8381534
  21. Transcript cleavage factors from E. coli.
    Cell. 1993 Feb 12;72(3):459-66 PMID: 8431948
  22. Elongation factor SII-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein.
    Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1917-21 PMID: 8446609
  23. SII-facilitated transcript cleavage in RNA polymerase II complexes stalled early after initiation occurs in primarily dinucleotide increments.
    J Biol Chem. 1993 Jun 15;268(17):12864-73 PMID: 8509420
  24. The increment of SII-facilitated transcript cleavage varies dramatically between elongation competent and incompetent RNA polymerase II ternary complexes.
    J Biol Chem. 1993 Jun 15;268(17):12874-85 PMID: 8509421
  25. Mechanism of DmS-II-mediated pause suppression by Drosophila RNA polymerase II.
    J Biol Chem. 1993 Sep 5;268(25):18762-70 PMID: 7689559
  26. Multiple RNA polymerase conformations and GreA: control of the fidelity of transcription.
    Science. 1993 Nov 5;262(5135):867-73 PMID: 8235608
  27. Purified yeast RNA polymerase II reads through intrinsic blocks to elongation in response to the yeast TFIIS analogue, P37.
    J Biol Chem. 1994 Jan 14;269(2):936-43 PMID: 8288647
  28. Hydrolytic cleavage of nascent RNA in RNA polymerase III ternary transcription complexes.
    J Biol Chem. 1994 Jan 21;269(3):2299-306 PMID: 7507490
  29. Discontinuous mechanism of transcription elongation.
    Science. 1994 Aug 5;265(5173):793-6 PMID: 8047884
  30. The active site of RNA polymerase II participates in transcript cleavage within arrested ternary complexes.
    Proc Natl Acad Sci U S A. 1994 Aug 16;91(17):8057-61 PMID: 8058756
  31. A model for transcription termination by RNA polymerase I.
    Cell. 1994 Nov 4;79(3):527-34 PMID: 7954818
  32. Coupling between transcription termination and RNA polymerase inchworming.
    Cell. 1995 May 5;81(3):351-7 PMID: 7736587
  33. Transcription termination of RNA polymerase I due to a T-rich element interacting with Reb1p.
    Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9781-5 PMID: 7568217
  34. The release element of the yeast polymerase I transcription terminator can function independently of Reb1p.
    Mol Cell Biol. 1995 Nov;15(11):5929-36 PMID: 7565745
  35. The RNA polymerase II elongation complex.
    FASEB J. 1995 Nov;9(14):1419-28 PMID: 7589983
  36. Identification of two different RNase H activities associated with yeast RNA polymerase A.
    J Biol Chem. 1979 Nov 10;254(21):10920-4 PMID: 387760
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.23.12914
Email
im4@ix.urz.uni-heidelberg.de
Published
1996-11-12
Pages
12914-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24020
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.23.12914 PMC Full Text View journal details
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