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PMID: 89073 Published · ppublish English Comparative Study Journal Article

Effects of alkylating agents on lymphocytes from controls and from patients with Fanconi's anemia. Studies of sister chromatid exchanges, chromosome aberrations, and kinetics of cell division.

Human genetics ·Vol. 49 ·No. 1 ·1979-05-23 ·Pages 41-50

Novotná B, Goetz P, Surkova NI

Abstract

The frequency of sister chromatid exchanges (SCE) and chromosome aberrations and the dynamics of cell division in peripheral blood lymphocytes of four patients with Fanconi's anemia were studied after in vitro exposure to alkylating agents TEPA and mitomycin. SCE frequency was significantly increased even after very low doses of mutagens, while chromosome aberrations were significantly increased only after high doses (0.160 micrograms/ml mitomycin and 10(-5) M TEPA). The responses of Fanconi's anemia cells and control cells did not differ significantly. The increased frequency of both SCE and chromosome aberrations was accompanied by gradual delay of cell division, which was most conspicuous in cells from patients with Fanconi's anemia.

MeSH Terms
Anemia, Aplastic/blood Azirines/pharmacology Cell Division/drug effects Child Chromatids/drug effects Chromosome Aberrations Crossing Over, Genetic/drug effects Fanconi Anemia/blood,genetics Humans Lymphocytes/drug effects,ultrastructure Mitomycins/pharmacology Triethylenephosphoramide/pharmacology
Chemicals
Azirines Mitomycins Triethylenephosphoramide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Novotná B
Goetz P
Surkova N I
References (18)
18 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00277685
Published
1979-05-23
Pages
41-50
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00277685 View journal details
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