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PMID: 8894980 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Surface Charges of K channels. Effects of strontium on five cloned channels expressed in Xenopus oocytes.

The Journal of general physiology ·Vol. 108 ·No. 4 ·1996-10-00 ·Pages 325-32

Elinder F, Madeja M, Arhem P

Abstract

The effects of strontium (Sr2+; 7-50 mM) on five different cloned rat K channels (Kv1.1, Kv1.5, Kv1.6, Kv2.1, and Kv3.4), expressed in oocytes of Xenopus laevis, were investigated with a two-electrode voltage clamp technique. The main effect was a shift of the Gk(V) curve along the potential axis, different in size for the different channels. Kv1.1 was shifted most and Kv3.4 least, 21 and 8 mV, respectively, at 50 mM. The effect was interpreted in terms of screening of fixed surface charges. The estimated charge densities ranged from -0.37 (Kv1.1) to -0.11 (Kv3.4) e nm-2 and showed good correlation with the total net charge of the extracellularly located amino acid residues of the channel as well as with the charge of a specific region (the loop between the S5 segment and the pore forming segment). The estimated surface potentials were found to be linearly related to the activation midpoint potential, suggesting a functional role for the surface charges.

MeSH Terms
Amino Acid Sequence Animals Cloning, Molecular Electric Conductivity Electrophysiology Female Oocytes/metabolism Potassium Channels/drug effects,genetics,physiology Rats Strontium/pharmacology Xenopus laevis
Chemicals
Potassium Channels Strontium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elinder F
Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.
Madeja M
Arhem P
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.108.4.325
Published
1996-10-00
Pages
325-32
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2229333
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.108.4.325 PMC Full Text View journal details
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