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PMID: 8879249 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of genes encoding subunits of the trehalose synthase complex in Saccharomyces cerevisiae: novel variations of STRE-mediated transcription control?

Molecular & general genetics : MGG ·Vol. 252 ·No. 4 ·1996-09-25 ·Pages 470-82

Winderickx J, de Winde JH, Crauwels M, Hino A, Hohmann S, Van Dijck P, Thevelein JM

Abstract

Saccharomyces cerevisiae cells show under suboptimal growth conditions a complex response that leads to the acquisition of tolerance to different types of environmental stress. This response is characterised by enhanced expression of a number of genes which contain so-called stress-responsive elements (STREs) in their promoters. In addition, the cells accumulate under suboptimal conditions the putative stress protectant trehalose. In this work, we have examined the expression of four genes encoding subunits of the trehalose synthase complex, GGS1/TPS1, TPS2, TPS3 and TSL1. We show that expression of these genes is coregulated under stress conditions. Like for many other genes containing STREs, expression of the trehalose synthase genes is also induced by heat and osmotic stress and by nutrient starvation, and negatively regulated by the Ras-cAMP pathway. However, during fermentative growth only TSL1 shows an expression pattern like that of the STRE-controlled genes CTT1 and SSA3, while expression of the three other trehalose synthase genes is only transiently down-regulated. This difference in expression might be related to the known requirement of trehalose biosynthesis for the control of yeast glycolysis and hence for fermentative growth. We conclude that the mere presence in the promoter of (an) active STRE(s) does not necessarily imply complete coregulation of expression. Additional mechanisms appear to fine tune the activity of STREs in order to adapt the expression of the downstream genes to specific requirements.

MeSH Terms
Blotting, Northern Cell Division/genetics Cyclic AMP-Dependent Protein Kinases/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Down-Regulation Enzyme Activation Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Fungal Glucose/metabolism Glucosyltransferases/biosynthesis,genetics,metabolism HSP70 Heat-Shock Proteins/genetics Multienzyme Complexes/genetics,metabolism Phosphoric Monoester Hydrolases/genetics,metabolism Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Transcription Factor AP-1/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic beta-Galactosidase/genetics,metabolism
Chemicals
CAD1 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins HSP70 Heat-Shock Proteins Multienzyme Complexes SSA3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factor AP-1 Transcription Factors YAP1 protein, S cerevisiae Glucosyltransferases trehalose synthase trehalose-6-phosphate synthase trehalose-6-phosphate synthase-phosphatase complex Cyclic AMP-Dependent Protein Kinases Phosphoric Monoester Hydrolases beta-Galactosidase Glucose
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Winderickx J
Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Leuven-Heverlee, Belgium.
de Winde J H
Crauwels M
Hino A
Hohmann S
Van Dijck P
Thevelein J M
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02173013
Published
1996-09-25
Pages
470-82
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02173013
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