Home LiteratureArticle Details
PMID: 7788713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of glucose influx into glycolysis and pleiotropic effects studied in different isogenic sets of Saccharomyces cerevisiae mutants in trehalose biosynthesis.

Current genetics ·Vol. 27 ·No. 2 ·1995-01-00 ·Pages 110-22

Neves MJ, Hohmann S, Bell W, Dumortier F, Luyten K, Ramos J, Cobbaert P, de Koning W, Kaneva Z, Thevelein JM

Abstract

The GGS1/TPS1 gene of the yeast Saccharomyces cerevisiae encodes the trehalose-6-phosphate synthase subunit of the trehalose synthase complex. Mutants defective in GGS1/TPS1 have been isolated repeatedly and they showed variable pleiotropic phenotypes, in particular with respect to trehalose content, ability to grow on fermentable sugars, glucose-induced signaling and sporulation capacity. We have introduced the fdp1, cif1, byp1 and glc6 alleles and the ggs1/tps1 deletion into three different wild-type strains, M5, SP1 and W303-1A. This set of strains will aid further studies on the molecular basis of the complex pleiotropic phenotypes of ggs1/tps1 mutants. The phenotypes conferred by specific alleles were clearly dependent on the genetic background and also differed for some of the alleles. Our results show that the lethality caused by single gene deletion in one genetic background can become undetectable in another background. The sporulation defect of ggs1/tps1 diploids was neither due to a deficiency in G1 arrest, nor to the inability to accumulate trehalose. Ggs1/tps1 delta mutants were very sensitive to glucose and fructose, even in the presence of a 100-fold higher galactose concentration. Fifty-percent inhibition occurred at concentrations similar to the Km values of glucose and fructose transport. The inhibitory effect of glucose in the presence of a large excess of galactose argues against an overactive glycolytic flux as the cause of the growth defect. Deletion of genes of the glucose carrier family shifted the 50% growth inhibition to higher sugar concentrations. This finding allows for a novel approach to estimate the relevance of the many putative glucose carrier genes in S. cerevisiae. We also show that the GGS1/TPS1 gene product is not only required for the transition from respirative to fermentative metabolism but continuously during logarithmic growth on glucose, in spite of the absence of trehalose under such conditions.

Related Genes
GGS1/TPS1
MeSH Terms
Cell Division DNA/biosynthesis Fructose/metabolism Fructose-Bisphosphatase/metabolism Glucose/metabolism Glucose-6-Phosphate Glucosephosphates/metabolism Glucosyltransferases/genetics,metabolism Glycogen Synthase/metabolism Glycolysis Mutation Plasmids/metabolism Saccharomyces cerevisiae/enzymology,genetics,growth & development,physiology Spores, Fungal Trehalose/biosynthesis
Chemicals
Glucosephosphates Fructose Glucose-6-Phosphate DNA Trehalose Glucosyltransferases Glycogen Synthase trehalose-6-phosphate synthase Fructose-Bisphosphatase Glucose
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Neves M J
Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit te Leuven, Leuven-Heverlee, Belgium.
Hohmann S
Bell W
Dumortier F
Luyten K
Ramos J
Cobbaert P
de Koning W
Kaneva Z
Thevelein J M
References (48)
48 references, click to expand
  1. A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coli otsA mutant.
    FEMS Microbiol Lett. 1993 Feb 15;107(1):25-30 PMID: 8467996
  2. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  3. A yeast homologue of the bovine lens fibre MIP gene family complements the growth defect of a Saccharomyces cerevisiae mutant on fermentable sugars but not its defect in glucose-induced RAS-mediated cAMP signalling.
    EMBO J. 1991 Aug;10(8):2095-104 PMID: 1648479
  4. Characterization of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase of Saccharomyces cerevisiae.
    Eur J Biochem. 1989 Jul 1;182(3):613-20 PMID: 2546763
  5. Disruption of TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity.
    Eur J Biochem. 1993 Mar 1;212(2):315-23 PMID: 8444170
  6. Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae.
    Eur J Biochem. 1993 Sep 15;216(3):849-61 PMID: 8404905
  7. Isolation of a regulatory mutant of fructose-1,6-diphosphatase in Saccharomyces carlsbergensis.
    J Bacteriol. 1974 Mar;117(3):965-70 PMID: 4360542
  8. Saccharomyces carlsbergensis fdp mutant and futile cycling of fructose 6-phosphate.
    Mol Cell Biol. 1982 Aug;2(8):921-9 PMID: 6290872
  9. The control of trehalose biosynthesis in Saccharomyces cerevisiae: evidence for a catabolite inactivation and repression of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase.
    Yeast. 1991 Aug-Sep;7(6):575-87 PMID: 1662849
  10. Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1730-4 PMID: 6300872
  11. Characterization of a regulatory mutant of fructose 1,6-bisphosphatase in Saccharomyces carlsbergensis.
    Mol Gen Genet. 1977 Jul 7;154(1):61-6 PMID: 197389
  12. Low-affinity glucose carrier gene LGT1 of Saccharomyces cerevisiae, a homologue of the Kluyveromyces lactis RAG1 gene.
    Yeast. 1993 Dec;9(12):1373-7 PMID: 8154188
  13. Characterization of the 56-kDa subunit of yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation.
    Eur J Biochem. 1992 Nov 1;209(3):951-9 PMID: 1425702
  14. Molecular cloning of CIF1, a yeast gene necessary for growth on glucose.
    Yeast. 1992 Mar;8(3):183-92 PMID: 1315471
  15. Elevated recombination rates in transcriptionally active DNA.
    Cell. 1989 Feb 24;56(4):619-30 PMID: 2645056
  16. Heat shock induces enzymes of trehalose metabolism, trehalose accumulation, and thermotolerance in Schizosaccharomyces pombe, even in the presence of cycloheximide.
    FEBS Lett. 1990 Oct 29;273(1-2):107-10 PMID: 2146164
  17. Replication and segregation of an unstable plasmid in yeast.
    Plasmid. 1982 Jul;8(1):15-28 PMID: 6755509
  18. The RAS-adenylate cyclase pathway and cell cycle control in Saccharomyces cerevisiae.
    Antonie Van Leeuwenhoek. 1992 Aug;62(1-2):109-30 PMID: 1444331
  19. The HXT2 gene of Saccharomyces cerevisiae is required for high-affinity glucose transport.
    Mol Cell Biol. 1990 Nov;10(11):5903-13 PMID: 2233722
  20. Effects of heat shock on the level of trehalose and glycogen, and on the induction of thermotolerance in Neurospora crassa.
    FEBS Lett. 1991 May 20;283(1):19-22 PMID: 1645296
  21. Roles of multiple glucose transporters in Saccharomyces cerevisiae.
    Mol Cell Biol. 1993 Jan;13(1):638-48 PMID: 8417358
  22. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  23. The control of glycogen metabolism in yeast. 1. Interconversion in vivo of glycogen synthase and glycogen phosphorylase induced by glucose, a nitrogen source or uncouplers.
    Eur J Biochem. 1988 Jun 15;174(3):551-9 PMID: 2839334
  24. Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae.
    Mol Microbiol. 1993 May;8(5):927-43 PMID: 8355617
  25. Galactose transport in Saccharomyces cerevisiae. II. Characteristics of galactose uptake and exchange in galactokinaseless cells.
    J Bacteriol. 1970 Sep;103(3):671-8 PMID: 5474882
  26. Analysis of PFK3--a gene involved in particulate phosphofructokinase synthesis reveals additional functions of TPS2 in Saccharomyces cerevisiae.
    Yeast. 1994 Feb;10 (2):199-209 PMID: 8203161
  27. Relationship between low- and high-affinity glucose transport systems of Saccharomyces cerevisiae.
    J Bacteriol. 1988 Nov;170(11):5375-7 PMID: 3053662
  28. Trehalose-6-phosphate, a new regulator of yeast glycolysis that inhibits hexokinases.
    FEBS Lett. 1993 Aug 23;329(1-2):51-4 PMID: 8354408
  29. A deletion of the PDC1 gene for pyruvate decarboxylase of yeast causes a different phenotype than previously isolated point mutations.
    Curr Genet. 1989 Feb;15(2):75-81 PMID: 2568187
  30. Pedigree analysis of plasmid segregation in yeast.
    Cell. 1983 Oct;34(3):961-70 PMID: 6354471
  31. Carbohydrate accumulation during the sporulation of yeast.
    J Bacteriol. 1970 Jan;101(1):53-7 PMID: 5411756
  32. Putting the HO gene to work: practical uses for mating-type switching.
    Methods Enzymol. 1991;194:132-46 PMID: 2005783
  33. In yeast, RAS proteins are controlling elements of adenylate cyclase.
    Cell. 1985 Jan;40(1):27-36 PMID: 2981630
  34. Investigation of the relationship between sst1 and fdp mutations in yeast and their effect on trehalose synthesis.
    Braz J Med Biol Res. 1985;18(4):447-54 PMID: 2942205
  35. The growth and signalling defects of the ggs1 (fdp1/byp1) deletion mutant on glucose are suppressed by a deletion of the gene encoding hexokinase PII.
    Curr Genet. 1993;23(4):281-9 PMID: 8467527
  36. Control of the yeast cell cycle by protein synthesis.
    Exp Cell Res. 1982 Nov;142(1):69-78 PMID: 6754401
  37. Fructose-1,6-diphosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting and non fermenting yeasts.
    Arch Mikrobiol. 1971;76(2):132-8 PMID: 4324161
  38. The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein.
    Proc Natl Acad Sci U S A. 1988 Apr;85(7):2130-4 PMID: 3281163
  39. Carbohydrate metabolism during ascospore development in yeast.
    J Bacteriol. 1974 Apr;118(1):8-14 PMID: 4595206
  40. The YDp plasmids: a uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae.
    Yeast. 1991 Jul;7(5):475-7 PMID: 1897313
  41. Phosphorus-31 nuclear magnetic resonance studies of wild-type and glycolytic pathway mutants of Saccharomyces cerevisiae.
    Biochemistry. 1979 Oct 16;18(21):4487-99 PMID: 40590
  42. Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae.
    Genetics. 1994 Feb;136(2):485-503 PMID: 8150278
  43. Glucose-induced regulatory defects in the Saccharomyces cerevisiae byp1 growth initiation mutant and identification of MIG1 as a partial suppressor.
    J Bacteriol. 1992 Jun;174(12):4183-8 PMID: 1597433
  44. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
    Gene. 1988 Dec 30;74(2):527-34 PMID: 3073106
  45. The HXT1 gene product of Saccharomyces cerevisiae is a new member of the family of hexose transporters.
    Mol Cell Biol. 1991 Jul;11(7):3804-13 PMID: 2046678
  46. Identification of extragenic suppressors of the cif1 mutation in Saccharomyces cerevisiae.
    Curr Genet. 1994 Feb;25(2):89-94 PMID: 8087890
  47. Yeast sugar transporters.
    Crit Rev Biochem Mol Biol. 1993;28(4):259-308 PMID: 8403984
  48. The SNF3 gene is required for high-affinity glucose transport in Saccharomyces cerevisiae.
    J Bacteriol. 1987 Apr;169(4):1656-62 PMID: 3549699
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00313424
Published
1995-01-00
Pages
110-22
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00313424 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2