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PMID: 5474882 Published · ppublish English Journal Article

Galactose transport in Saccharomyces cerevisiae. II. Characteristics of galactose uptake and exchange in galactokinaseless cells.

Journal of bacteriology ·Vol. 103 ·No. 3 ·1970-09-00 ·Pages 671-8

Kou SC, Christensen MS, Cirillo VP

Abstract

The characteristics of the inducible galactose system in Saccharomyces cerevisiae were studied by using the nonmetabolized galactose analogues, l-arabinose and d-fucose, and galactokinaseless and transportless mutants. Induced wild-type cells transport l-arabinose by facilitated diffusion. Transportless cells transport neither galactose nor l-arabinose above the noninduced rate, whereas galactokinaseless cells transport galactose l-arabinose and d-fucose by facilitated diffusion. Determination of unidirectional rate of (14)C-labeled galactose uptake by preloaded galactokinaseless cells, containing a large unlabeled free-galactose pool, showed that the rate of galactose uptake by facilitated diffusion is greater than the rate of galactose metabolism at similar external galactose concentrations.

MeSH Terms
Arabinose/metabolism Biological Transport Carbon Isotopes Chromatography Culture Media Diffusion Filtration Fucose/metabolism Galactose/metabolism Genetics, Microbial Iodoacetates/pharmacology Mutation Phosphotransferases/metabolism Saccharomyces/enzymology,growth & development,metabolism Stereoisomerism Uranium/pharmacology
Chemicals
Carbon Isotopes Culture Media Iodoacetates Fucose Uranium Arabinose Phosphotransferases Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kou S C
Christensen M S
Cirillo V P
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21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.103.3.671-678.1970
Published
1970-09-00
Pages
671-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248142
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.103.3.671-678.1970 PMC Full Text View journal details
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