Galactose transport in Saccharomyces cerevisiae. 3. Characteristics of galactose uptake in transferaseless cells: evidence against transport-associated phosphorylation.

Journal of bacteriology ·Vol. 103 ·No. 3 ·1970-09-00 ·Pages 679-85

Kuo SC, Cirillo VP

Abstract

The characteristics of the inducible galactose transport system in bakers' yeast were studied in uridine diphosphate, galactose-1-phosphate uridylyl-transferaseless cells. Transferaseless cells transport galactose at the same initial rate as wild-type cells and accumulate a mixture of free galactose and galactose-1-phosphate. The addition of (14)C-labeled galactose to cells preloaded with unlabeled galactose and galactose-1-phosphate results in a higher rate of labeling of the free-sugar pool than of the galactose-1-phosphate pool. These results support other evidence that galactose uptake in bakers' yeast is a carrier-mediated, facilitated diffusion and that phosphorylation is an intracellular event after uptake of the free sugar.

MeSH Terms

Biological Transport Carbon Isotopes Chromatography Culture Media Diffusion Filtration Galactose/metabolism Genetics, Microbial Hexosephosphates/metabolism Models, Theoretical Mutation Nucleotidyltransferases/metabolism Oxidative Phosphorylation Saccharomyces/enzymology,growth & development,metabolism

Chemicals

Carbon Isotopes Culture Media Hexosephosphates Nucleotidyltransferases Galactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kuo S C

Cirillo V P

References (23)

23 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.103.3.679-685.1970

Published

1970-09-00

Pages

679-85

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248143

Subset

External Links

PubMed source DOI: 10.1128/jb.103.3.679-685.1970 PMC Full Text View journal details

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Galactose transport in Saccharomyces cerevisiae. 3. Characteristics of galactose uptake in transferaseless cells: evidence against transport-associated phosphorylation.

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