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PMID: 2967122 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of the trehalose-6-phosphate synthase complex in Saccharomyces. I. Interconversion of forms by phosphorylation.

Current genetics ·Vol. 11 ·No. 6-7 ·1987-00-00 ·Pages 459-65

Panek AC, de Araujo PS, Moura Neto V, Panek AD

Abstract

Trehalose-6-phosphate synthase is another example of an enzyme of carbohydrate metabolism, in Saccharomyces, which could be regulated by interconversion of forms. Deactivation was mediated both in vivo and in vitro by a cyclic AMP-dependent protein kinase. Reversibility of this process was obtained by a phosphatase treatment leading to an increase in activity. The phosphorylated, less active form of the enzyme proved to be more susceptible to activation by ATP.Mg. Mutants with well defined lesions in the cyclic AMP-dependent protein kinase system were used to corroborate our findings of a possible regulatory mechanism of trehalose-6-phosphate synthase activity by interconversion of forms.

MeSH Terms
Genes Genes, Fungal Genes, Regulator Glucosyltransferases/genetics,metabolism Kinetics Multienzyme Complexes/genetics Phosphorylation Protein Kinases/metabolism Saccharomyces cerevisiae/enzymology,genetics Species Specificity
Chemicals
Multienzyme Complexes Glucosyltransferases trehalose-6-phosphate synthase Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Panek A C
Departamento de Bioquímica, Instituto de Química, CCMN, Universidade Federal do Rio de Janeiro, Brasil.
de Araujo P S
Moura Neto V
Panek A D
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00384607
Published
1987-00-00
Pages
459-65
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00384607 View journal details
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