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PMID: 8852839 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

New mutants of Saccharomyces cerevisiae affected in the transport of proteins from the endoplasmic reticulum to the Golgi complex.

Genetics ·Vol. 142 ·No. 2 ·1996-02-00 ·Pages 393-406

Wuestehube LJ, Duden R, Eun A, Hamamoto S, Korn P, Ram R, Schekman R

Abstract

We have isolated new temperature-sensitive mutations in five complementation groups, sec31-sec35, that are defective in the transport of proteins from the endoplasmic reticulum (ER) to the Golgi complex. The sec31-sec35 mutants and additional alleles of previously identified sec and vacuolar protein sorting (vps) genes were isolated in a screen based on the detection of alpha-factor precursor in yeast colonies replicated to and lysed on nitrocellulose filters. Secretory protein precursors accumulated in sec31-sec35 mutants at the nonpermissive temperature were core-glycosylated but lacked outer chain carbohydrate, indicating that transport was blocked after translocation into the ER but before arrival in the Golgi complex. Electron microscopy revealed that the newly identified sec mutants accumulated vesicles and membrane structures reminiscent of secretory pathway organelles. Complementation analysis revealed that sec32-1 is an allele of BOS1, a gene implicated in vesicle targeting to the Golgi complex, and sec33-1 is an allele of RET1, a gene that encodes the alpha subunit of coatomer.

MeSH Terms
Biological Transport Endoplasmic Reticulum/metabolism Epistasis, Genetic Fungal Proteins/metabolism Gene Expression Regulation, Fungal Golgi Apparatus/metabolism Immunoblotting Membrane Proteins/metabolism Mutation Protein Precursors Saccharomyces cerevisiae/genetics
Chemicals
Fungal Proteins Membrane Proteins Protein Precursors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wuestehube L J
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Duden R
Eun A
Hamamoto S
Korn P
Ram R
Schekman R
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/142.2.393
Published
1996-02-00
Pages
393-406
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206974
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/142.2.393 PMC Full Text View journal details
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