Home LiteratureArticle Details
PMID: 8764062 Published · ppublish English Journal Article

Interaction of human immunodeficiency virus type 1 Tat with a unique site of TFIID inhibits negative cofactor Dr1 and stabilizes the TFIID-TFIIA complex.

Journal of virology ·Vol. 70 ·No. 8 ·1996-08-00 ·Pages 5503-10

Kashanchi F, Khleif SN, Duvall JF, Sadaie MR, Radonovich MF, Cho M, Martin MA, Chen SY, Weinmann R, Brady JN

Abstract

We have previously reported the direct physical interaction between the human immunodeficiency virus (HIV) type I Tat protein and the basal transcription factor TBP/TFIID. Affinity chromatography demonstrated that wild-type Tat, but not a transactivation mutant of Tat, was capable of depleting TBP/TFIID from cell extracts. These experiments represented the first demonstration of a basal transcription factor that binds, in an activation-dependent manner, to Tat. We now report that the Tat-TBP interaction can be detected in HIV type 1-infected cells. The domain of TBP interacting with Tat has been mapped from amino acids 163 to 196 by using deletion and site-specific mutants of TBP. This domain of TBP, which includes the HI and S2 domains, is distinct from the H2 binding site for other activator proteins, such as E1A. The interaction of Tat with TFIID regulates the binding of accessory proteins to TFIID. Tat stabilizes the interaction of TFIID with TFIIA in a gel shift assay. In addition, Tat competes for Dr1 interaction with TBP. Our results suggest that the basal transcription factor TBP/TFIID represents an important regulatory molecule in HIV transcription.

MeSH Terms
Cell Line Gene Products, tat/metabolism HIV Infections/metabolism,virology HIV-1/metabolism Humans Peptide Mapping Phosphoproteins/metabolism Protein Binding Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors/metabolism tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat Phosphoproteins Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors down-regulator of transcription 1 tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Kashanchi F
Laboratory of Molecular Virology, National Cancer Institute, Bethesda, Maryland 20892, USA.
Khleif S N
Duvall J F
Sadaie M R
Radonovich M F
Cho M
Martin M A
Chen S Y
Weinmann R
Brady J N
References (58)
58 references, click to expand
  1. Cell-to-cell spread of HIV-1 occurs within minutes and may not involve the participation of virus particles.
    Virology. 1992 Feb;186(2):712-24 PMID: 1370739
  2. The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1.
    Cell. 1992 Mar 6;68(5):965-76 PMID: 1547496
  3. Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription.
    Cell. 1992 Aug 7;70(3):477-89 PMID: 1339312
  4. Electroporation of viral transactivator proteins into lymphocyte suspension cells.
    Nucleic Acids Res. 1992 Sep 11;20(17):4673-4 PMID: 1408778
  5. The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB.
    Cell. 1992 Dec 11;71(6):1055-64 PMID: 1458536
  6. Analysis of Tat transactivation of human immunodeficiency virus transcription in vitro.
    Gene Expr. 1992;2(4):391-407 PMID: 1282057
  7. Human immunodeficiency viruses containing heterologous enhancer/promoters are replication competent and exhibit different lymphocyte tropisms.
    J Virol. 1993 Feb;67(2):743-52 PMID: 8419644
  8. The type 1 human immunodeficiency virus Tat binding protein is a transcriptional activator belonging to an additional family of evolutionarily conserved genes.
    Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):138-42 PMID: 8419915
  9. Transcriptional activation by simian virus 40 large T antigen: interactions with multiple components of the transcription complex.
    Mol Cell Biol. 1993 Feb;13(2):961-9 PMID: 8423815
  10. TBP, a universal eukaryotic transcription factor?
    Genes Dev. 1993 Jul;7(7B):1291-308 PMID: 8330735
  11. Functional interaction of adenovirus E1A with holo-TFIID.
    Genes Dev. 1993 Sep;7(9):1810-23 PMID: 8370528
  12. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  13. Eukaryotic gene transcription with purified components.
    Methods Enzymol. 1983;101:582-98 PMID: 6888276
  14. Transcription initiation by RNA polymerase II in vitro. At least two nucleotides must be added to form a stable ternary complex.
    J Biol Chem. 1987 Jan 5;262(1):289-97 PMID: 2432060
  15. Transcription initiation by RNA polymerase II in vitro. Properties of preinitiation, initiation, and elongation complexes.
    J Biol Chem. 1987 Jan 5;262(1):298-304 PMID: 2432061
  16. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE.
    J Biol Chem. 1987 Mar 5;262(7):3310-21 PMID: 3029109
  17. Functional steps in transcription initiation and reinitiation from the major late promoter in a HeLa nuclear extract.
    J Biol Chem. 1987 Mar 15;262(8):3452-61 PMID: 2434502
  18. The HIV tat gene induces dermal lesions resembling Kaposi's sarcoma in transgenic mice.
    Nature. 1988 Oct 13;335(6191):606-11 PMID: 2845275
  19. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  20. Promoter specificity and modulation of RNA polymerase II transcription.
    FASEB J. 1989 Apr;3(6):1723-33 PMID: 2649403
  21. HIV-1 Tat protein increases transcriptional initiation and stabilizes elongation.
    Cell. 1989 Oct 20;59(2):283-92 PMID: 2553266
  22. Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4509-13 PMID: 2141169
  23. Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID.
    Nature. 1990 Jun 28;345(6278):783-6 PMID: 2193231
  24. Functional domains and upstream activation properties of cloned human TATA binding protein.
    Science. 1990 Jun 29;248(4963):1625-30 PMID: 2363050
  25. Cloning of a transcriptionally active human TATA binding factor.
    Science. 1990 Jun 29;248(4963):1646-50 PMID: 2194289
  26. Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIID.
    Cell. 1990 Jun 29;61(7):1179-86 PMID: 2194666
  27. Mechanism of transcriptional activation by Sp1: evidence for coactivators.
    Cell. 1990 Jun 29;61(7):1187-97 PMID: 2194667
  28. Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID).
    Nature. 1990 Jul 26;346(6282):387-90 PMID: 2374612
  29. HIV-1 Tat protein trans-activates transcription in vitro.
    Cell. 1990 Nov 16;63(4):791-802 PMID: 2225077
  30. Mechanism of action of an acidic transcriptional activator in vitro.
    Cell. 1991 Mar 8;64(5):971-81 PMID: 2001592
  31. The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):4045-9 PMID: 2023953
  32. Activation of class II gene transcription by regulatory factors is potentiated by a novel activity.
    Cell. 1991 Sep 6;66(5):981-93 PMID: 1889091
  33. Host range, replicative, and cytopathic properties of human immunodeficiency virus type 1 are determined by very few amino acid changes in tat and gp120.
    J Virol. 1991 Dec;65(12):6931-41 PMID: 1658383
  34. The HIV-1 Tat protein activates transcription from an upstream DNA-binding site: implications for Tat function.
    Genes Dev. 1991 Dec;5(12B):2496-507 PMID: 1752440
  35. HIV-1 Tat protein promotes formation of more-processive elongation complexes.
    EMBO J. 1991 Dec;10(13):4189-96 PMID: 1756726
  36. In vitro and in vivo binding of human immunodeficiency virus type 1 Tat protein and Sp1 transcription factor.
    J Virol. 1993 Oct;67(10):6224-33 PMID: 7690421
  37. Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB.
    Cell. 1993 Nov 5;75(3):519-30 PMID: 8221891
  38. Eukaryotic activators function during multiple steps of preinitiation complex assembly.
    Nature. 1993 Dec 9;366(6455):531-6 PMID: 8255291
  39. Direct interaction of human TFIID with the HIV-1 transactivator tat.
    Nature. 1994 Jan 20;367(6460):295-9 PMID: 8121496
  40. The zinc finger region of the adenovirus E1A transactivating domain complexes with the TATA box binding protein.
    Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2488-92 PMID: 8146144
  41. Second-site long terminal repeat (LTR) revertants of replication-defective human immunodeficiency virus: effects of revertant TATA box motifs on virus infectivity, LTR-directed expression, in vitro RNA synthesis, and binding of basal transcription factors TFIID and TFIIA.
    J Virol. 1994 May;68(5):3298-307 PMID: 8151790
  42. Transcriptional activation of minimal HIV-1 promoter by ORF-1 protein expressed from the SalI-L fragment of human herpesvirus 6.
    Virology. 1994 May 15;201(1):95-106 PMID: 8178493
  43. Transcriptional elongation by RNA polymerase II is stimulated by transactivators.
    Cell. 1994 Jun 3;77(5):749-59 PMID: 8205623
  44. Interaction of the Dr1 inhibitory factor with the TATA binding protein is disrupted by adenovirus E1A.
    Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6279-82 PMID: 8022773
  45. Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor.
    Cell. 1994 Oct 7;79(1):107-17 PMID: 7923369
  46. Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53.
    Mol Cell Biol. 1994 Oct;14(10):7013-24 PMID: 7935417
  47. Synergy between basic fibroblast growth factor and HIV-1 Tat protein in induction of Kaposi's sarcoma.
    Nature. 1994 Oct 20;371(6499):674-80 PMID: 7935812
  48. Differential regulation of RNA polymerases I, II, and III by the TBP-binding repressor Dr1.
    Science. 1994 Oct 21;266(5184):448-50 PMID: 7939686
  49. Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription.
    Genes Dev. 1994 Oct 1;8(19):2324-35 PMID: 7958899
  50. Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription.
    Genes Dev. 1994 Oct 1;8(19):2336-48 PMID: 7958900
  51. Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, relieving p53-mediated transcriptional repression.
    Mol Cell Biol. 1995 Jan;15(1):227-34 PMID: 7799929
  52. Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators.
    Science. 1995 Jan 27;267(5197):531-6 PMID: 7824954
  53. Novel mechanism and factor for regulation by HIV-1 Tat.
    EMBO J. 1995 Jan 16;14(2):321-8 PMID: 7835343
  54. Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor.
    J Virol. 1995 Mar;69(3):1612-20 PMID: 7853496
  55. In vitro interaction of the human immunodeficiency virus type 1 Tat transactivator and the general transcription factor TFIIB with the cellular protein TAP.
    J Virol. 1995 May;69(5):3017-23 PMID: 7707528
  56. Evidence for functional interaction between the HIV-1 Tat transactivator and the TATA box binding protein in vivo.
    J Mol Biol. 1995 Jul 7;250(2):169-80 PMID: 7608968
  57. Transactivation of the human T-cell lymphotropic virus type 1 Tax1-responsive 21-base-pair repeats requires Holo-TFIID and TFIIA.
    J Virol. 1995 Aug;69(8):5077-86 PMID: 7609077
  58. HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors.
    Genes Dev. 1992 Apr;6(4):655-66 PMID: 1559613
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.8.5503-5510.1996
Published
1996-08-00
Pages
5503-10
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190508
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.8.5503-5510.1996 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2