Home LiteratureArticle Details

PMID: 875135 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Genome RNAs and polypeptides of reovirus serotypes 1, 2, and 3.

Journal of virology ·Vol. 22 ·No. 3 ·1977-06-00 ·Pages 726-33

Ramig RF, Cross RK, Fields BN

Abstract

The virus-specific double-stranded genome RNA and polypeptides present in virions and cells infected with the three mammalian reovirus serotypes have been examined by co-electrophoresis in several different polyacrylamide gel systems. The double-stranded RNA and polypeptide species previously described for type 3 Dearing were found to have corresponding species in the other serotypes examined. In each serotype several RNA and polypeptide species were found to have different electrophoretic mobilities from the corresponding RNA or polypeptide species of type 3 Dearing. The combination of electrophoretic variants among the RNAs and polypeptides of the reovirus serotypes gave electrophoretic markers in all 10 of the reovirus genes. The usefulness of these electrophoretic markers in "mapping" the reovirus genome is discussed.

MeSH Terms

L Cells Mammalian orthoreovirus 3/analysis Molecular Weight Nucleic Acid Conformation Peptides/analysis Protein Conformation RNA, Viral/analysis Reoviridae/analysis Viral Proteins/analysis

Chemicals

Peptides RNA, Viral Viral Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ramig R F

Cross R K

Fields B N

References (28)

28 references, click to expand

Reoviruses in animals other than man.
Ann N Y Acad Sci. 1962 Nov 30;101:461-5 PMID: 13974842
Reoviruses. A new group of respiratory and enteric viruses formerly classified as ECHO type 10 is described.
Science. 1959 Nov 20;130(3386):1387-9 PMID: 14440555
Serologic grouping of reoviruses by hemagglutination-inhibition.
Am J Hyg. 1960 Mar;71:242-9 PMID: 14438891
Use of an aberrant polypeptide as a marker in three-factor crosses: further evidence for independent reassortment as the mechanism of recombination between temperature-sensitive mutants of reovirus type 3.
Virology. 1976 Oct 15;74(2):345-62 PMID: 982831
Essential and nonessential noncapsid reovirus proteins.
Virology. 1971 Sep;45(3):716-23 PMID: 5166483
Translation of reovirus messenger RNAs synthetesized in vitro into reovirus polypeptides by several mammalian cell-free extracts.
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2649-53 PMID: 4506788
Temperature-sensitive mutants of reovirus type 3: studies on the synthesis of viral RNA.
Virology. 1972 Dec;50(3):799-809 PMID: 4674371
Hybridization and sedimentation studies on "early" and "late" vaccinia messenger RNA.
J Mol Biol. 1967 Aug 14;27(3):395-419 PMID: 6049681
Studies on reovirus RNA. II. Characterization of reovirus messenger RNA and of the genome RNA segments from which it is transcribed.
J Mol Biol. 1967 Oct 14;29(1):19-26 PMID: 6055333
Polypeptide components of virions, top component and cores of reovirus type 3.
Virology. 1969 Dec;39(4):791-810 PMID: 4311639
Molecular hybridization studies on the relationships between different serotypes of bluetongue virus and on the difference between the virulent and attenuated strains of the same serotype.
Onderstepoort J Vet Res. 1973 Sep;40(3):93-103 PMID: 4132922
A temperature-sensitive mutant of vesicular stomatitis virus with two abnormal virus proteins.
J Gen Virol. 1974 Apr;23(1):97-106 PMID: 4364877
Comparison of the capsid polypeptides of various bluetongue virus serotypes.
Intervirology. 1974;3(1-2):47-53 PMID: 4373408
Purification and properties of poliovirus double-stranded ribonucleic acid.
J Mol Biol. 1966 Jul;18(3):421-8 PMID: 4290740
Evidence for a glycoprotein in reovirus.
Virology. 1976 Jul 15;72(2):308-21 PMID: 948864
Temperature-sensitive mutants of reovirus type 3: evidence for aberrant mu 1 and mu 2 polypeptide species.
J Virol. 1976 Jul;19(1):174-9 PMID: 950685
Reovirus-specific polypeptides: analysis using discontinuous gel electrophoresis.
J Virol. 1976 Jul;19(1):162-73 PMID: 950684
Synthesis of all the gene products of the reovirus genome in vivo and in vitro.
Cell. 1975 Feb;4(2):173-80 PMID: 1125978
Evidence for phosphoproteins in reovirus.
Virology. 1975 Apr;64(2):505-12 PMID: 1169848
Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes.
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2142-6 PMID: 1064882
Sequence homology studies between the double-stranded RNA genomes of cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus strains 1, 2 and 3.
Intervirology. 1974;4(2):91-8 PMID: 4615076
Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology.
Infect Immun. 1976 Jan;13(1):211-20 PMID: 175016
Differences in RNA patterns of influenza A viruses.
J Virol. 1976 Mar;17(3):876-84 PMID: 1255862
RNAs of influenza A, B, and C viruses.
J Virol. 1976 May;18(2):738-44 PMID: 944790
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.
J Virol. 1968 Oct;2(10):986-91 PMID: 5723704
Preparation of mammalian polyribosomes with the detergent Nonidet P-40.
Biochim Biophys Acta. 1967 Nov 21;149(1):302-4 PMID: 5582750
Studies on reovirus RNA. I. Characterization of reovirus genome RNA.
J Mol Biol. 1967 Oct 14;29(1):1-17 PMID: 6055334

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.22.3.726-733.1977

Published

1977-06-00

Pages

726-33

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515772

Subset

External Links

PubMed source DOI: 10.1128/JVI.22.3.726-733.1977 PMC Full Text View journal details

Analysis Services

Genome RNAs and polypeptides of reovirus serotypes 1, 2, and 3.

Contact

WeChat Official Account