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PMID: 950684 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Reovirus-specific polypeptides: analysis using discontinuous gel electrophoresis.

Journal of virology ·Vol. 19 ·No. 1 ·1976-07-00 ·Pages 162-73

Cross RK, Fields BN

Abstract

The electrophoretic analysis of reovirus-specific polypeptides in infected cells using a discontinuous gel system has allowed the resolution of additional viral-specific polypeptides, including one large-sized gamma3 and two (or possibly three) medium-sized (mu3, mu4, mu5(?)) species. The proteins designated mu0, sigma1, and sigma2 based on electrophoretic mobility in gel systems containing phosphate-urea correspond to mu4, sigma2, and sigma1, respectively, when analyzed in systems containing Tris-glycine. It is likely that protein modifications (phosphorylation and glycosylation) are responsible for at least some of these differences.

MeSH Terms
Acetone Buffers Chemical Precipitation Electrophoresis, Polyacrylamide Gel/methods Glycine Immune Sera L Cells Mammalian orthoreovirus 3/analysis Peptides/analysis Phosphates Reoviridae/analysis Tromethamine Urea Viral Proteins/analysis
Chemicals
Buffers Immune Sera Peptides Phosphates Viral Proteins Tromethamine Acetone Urea Glycine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cross R K
Fields B N
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30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.19.1.162-173.1976
Published
1976-07-00
Pages
162-73
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354843
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.19.1.162-173.1976 PMC Full Text View journal details
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