Reovirus-specific ribonucleic acid from polysomes of infected L cells.

Journal of virology ·Vol. 9 ·No. 1 ·1972-01-00 ·Pages 61-9

Ward R, Banerjee AK, LaFiandra A, Shatkin AJ

Abstract

Polysomes from reovirus-infected L cells were analyzed. Although all 10 transcription products of the viral genome were represented in polysomes and presumably can be translated, fewer than 10 reovirus-specific polypeptides were detected in infected cells. The 5'-terminal sequence of all species of reovirus ribonucleic acid (RNA) from polysomes was ppGpYp, as previously found for the genome double-stranded RNA and the in vitro single-stranded products of the virion-associated RNA polymerase. RNA isolated from both heavy (>30 ribosomes) and light (5 to 8 ribosomes) polysomes includes similar amounts of large, medium, and small classes of reovirus single-stranded RNA, suggesting that linkage of the genome segments observed in virions may occur at the single-strand stage of RNA replication.

MeSH Terms

Animals Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Electrophoresis, Disc Genetic Code Genetic Linkage L Cells/analysis,metabolism Mice Nucleic Acid Hybridization Peptide Chain Termination, Translational Peptides/analysis Phosphorus Isotopes RNA Nucleotidyltransferases/metabolism RNA, Ribosomal/analysis RNA, Viral/analysis,biosynthesis Reoviridae/analysis,enzymology,growth & development,metabolism Ribosomes/analysis Tritium Uridine/metabolism Viral Proteins/biosynthesis Virus Replication

Chemicals

Peptides Phosphorus Isotopes RNA, Ribosomal RNA, Viral Viral Proteins Tritium RNA Nucleotidyltransferases Uridine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ward R

Banerjee A K

LaFiandra A

Shatkin A J

References (26)

26 references, click to expand

In vitro synthesis of native myosin, actin, and tropomyosin from embryonic chick polyribosomes.
Proc Natl Acad Sci U S A. 1968 Feb;59(2):590-7 PMID: 5238987
Studies on reovirus RNA. I. Characterization of reovirus genome RNA.
J Mol Biol. 1967 Oct 14;29(1):1-17 PMID: 6055334
Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase.
J Virol. 1970 Jul;6(1):1-11 PMID: 5529847
Studies on the A-rich RNA of reovirus.
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1389-95 PMID: 5237873
Single-stranded, adenine-rich RNA from purified reoviruses.
Proc Natl Acad Sci U S A. 1968 Jan;59(1):246-53 PMID: 5242127
Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336-40 PMID: 5288383
The biochemistry of RNA bacteriophage replication.
Annu Rev Biochem. 1970;39:527-60 PMID: 4921696
RNA polymerase activity in purified reoviruses.
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462-9 PMID: 4179180
Asynchronous synthesis of the complementary strands of the reovirus genome.
Proc Natl Acad Sci U S A. 1971 Feb;68(2):505-8 PMID: 5277109
Properties of RNA transcriptase in reovirus subviral particles.
Proc Natl Acad Sci U S A. 1970 Jul;66(3):890-7 PMID: 5269250
Polypeptide components of virions, top component and cores of reovirus type 3.
Virology. 1969 Dec;39(4):791-810 PMID: 4311639
A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5 PMID: 5288382
Initiation of reovirus mRNA synthesis in vitro.
Nat New Biol. 1971 Apr 7;230(14):169-72 PMID: 4324352
Electron microscopy of RNA strands released from individual Reovirus particles.
J Mol Biol. 1968 May 28;34(1):137-47 PMID: 5760451
Collagen synthesis on polysomes in vivo and in vitro.
Nat New Biol. 1971 Jul 14;232(28):37-40 PMID: 5284450
THE SECONDARY STRUCTURE OF REOVIRUS RNA.
Proc Natl Acad Sci U S A. 1963 May;49(5):707-14 PMID: 16591092
An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321-5 PMID: 5288381
Preparation of mammalian polyribosomes with the detergent Nonidet P-40.
Biochim Biophys Acta. 1967 Nov 21;149(1):302-4 PMID: 5582750
Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores.
Virology. 1969 Dec;39(4):822-31 PMID: 5358831
Reovirus-directed ribonucleic acid synthesis in infected L cells.
J Virol. 1967 Feb;1(1):24-35 PMID: 4195926
Absence of adenine-rich ribonucleic acid from purified infectious reovirus 3.
J Virol. 1969 Nov;4(5):642-50 PMID: 4902632
Reovirus-specific polyribosomes in infected L-cells.
Science. 1966 Oct 28;154(3748):522-3 PMID: 5916947
Studies on the intracellular synthesis of reovirus-specified proteins.
Virology. 1970 Jul;41(3):501-18 PMID: 5463865
ACTINOMYCIN AND THE DIFFERENTIAL SYNTHESIS OF REOVIRUS AND L CELL RNA.
Biochem Biophys Res Commun. 1965 May 3;19:506-10 PMID: 14338999
Synthesis of reovirus ribonucleic acid in L cells.
J Bacteriol. 1965 Oct;90(4):936-45 PMID: 4158705
Demonstration that the same strand of reovirus genome RNA is transcribed in vitro and in vivo.
Virology. 1971 May;44(2):450-3 PMID: 5105773

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.1.61-69.1972

Published

1972-01-00

Pages

61-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356262

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.1.61-69.1972 PMC Full Text View journal details

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Reovirus-specific ribonucleic acid from polysomes of infected L cells.

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