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PMID: 8702718 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and characterization of a gene encoding a gut-enriched Krüppel-like factor expressed during growth arrest.

The Journal of biological chemistry ·Vol. 271 ·No. 33 ·1996-08-16 ·Pages 20009-17

Shields JM, Christy RJ, Yang VW

Abstract

A cDNA clone, named gut-enriched Krüppel-like factor (GKLF), was isolated from an NIH 3T3 library using a probe encoding the zinc finger region of the immediate-early transcription factor zif/268. The deduced GKLF amino acid sequence contains three tandem zinc fingers that are related to members of the Krüppel family of transcription factors. By indirect immunofluorescence, GKLF is localized to the cell nucleus. In cultured fibroblasts, GKLF mRNA is found in high levels in growth-arrested cells and is nearly undetectable in cells that are in the exponential phase of proliferation. The growth-arresting nature of GKLF is demonstrated by an inhibition of DNA synthesis in cells transfected with a GKLF-expressing plasmid construct. In the mouse, GKLF mRNA is present in select tissues and is most abundant in the colon, followed by the testis, lung, and small intestine. In situ hybridization experiments indicate that GKLF mRNA is enriched in epithelial cells located in the middle to upper crypt region of the colonic mucosa. Taken together, these results suggest that GKLF is potentially a negative regulator of cell growth in tissues such as the gut mucosa, where cell proliferation is intimately coupled to growth arrest and differentiation.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Base Sequence Binding Sites Cell Cycle Colon/metabolism Culture Media DNA/biosynthesis DNA-Binding Proteins/genetics Gene Expression Genes In Situ Hybridization Kruppel-Like Factor 4 Kruppel-Like Transcription Factors Mice Molecular Sequence Data Nuclear Proteins/genetics RNA, Messenger/genetics Sequence Alignment Sequence Homology, Amino Acid Tissue Distribution Transcription Factors/genetics Zinc Fingers
Chemicals
Culture Media DNA-Binding Proteins Klf4 protein, mouse Kruppel-Like Factor 4 Kruppel-Like Transcription Factors Nuclear Proteins RNA, Messenger Transcription Factors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shields J M
Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Christy R J
Yang V W
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
DOI
10.1074/jbc.271.33.20009
Published
1996-08-16
Pages
20009-17
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2330254
Subset
IM
Grants
NIDDK NIH HHS · P01 DK044484-100005 · United States
NCI NIH HHS · R01 CA084197 · United States
NIDDK NIH HHS · R01 DK052230 · United States
NIDDK NIH HHS · R01 DK052230-03 · United States
Databases
GENBANK
U20344
External Links
PubMed source DOI: 10.1074/jbc.271.33.20009 PMC Full Text View journal details
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