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PMID: 8692870 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel function for Myc: inhibition of C/EBP-dependent gene activation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 13 ·1996-06-25 ·Pages 6635-40

Mink S, Mutschler B, Weiskirchen R, Bister K, Klempnauer KH

Abstract

We have investigated the effect of the v-Myc oncoprotein on gene expression in myelomonocytic cells. We find that v-Myc dramatically down-regulates the expression of myelomonocytic-specific genes, such as the chicken mim-1 and lysozyme genes, both of which are known targets for C/EBP transcription factors. We present evidence that Myc downregulates these genes by inhibiting the function of C/EBP transcription factors. Detailed examination of the inhibitory mechanism shows that amino-terminal sequences of v-Myc, but not its DNA-binding domain, are required for the suppression of C/EBP-dependent transactivation. Our findings identify a new function for Myc and reveal a novel mechanism by which Myc affects the expression of other genes.

MeSH Terms
Acetyltransferases Animals CCAAT-Enhancer-Binding Proteins Cell Line Chickens DNA-Binding Proteins/genetics,physiology Down-Regulation Gene Expression Regulation/physiology Muramidase/genetics Nuclear Proteins/genetics Oncogene Protein p55(v-myc)/chemistry,physiology Proteins/genetics Quail Transcriptional Activation
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Nuclear Proteins Oncogene Protein p55(v-myc) Proteins Acetyltransferases mim-1 protein, Gallus gallus Muramidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mink S
Hans Spemann Laboratory, Max Planck for Immunobiology, Freiburg, Germany.
Mutschler B
Weiskirchen R
Bister K
Klempnauer K H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.13.6635
Published
1996-06-25
Pages
6635-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC39078
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.13.6635 PMC Full Text View journal details
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