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PMID: 8670903 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Btcd, a mouse protein that binds to curved DNA, can substitute in Escherichia coli for H-NS, a bacterial nucleoid protein.

The EMBO journal ·Vol. 15 ·No. 15 ·1996-08-01 ·Pages 3986-92

Timchenko T, Bailone A, Devoret R

Abstract

In an Escherichia coli mutant devoid of H-NS, a bacterial nucleoid protein, mouse protein Btcd was able to substitute for H-NS in two tested functions. It restored cell motility and repression of the expression of the bgl operon. Btcd1, a mutant Btcd protein deleted of its zinc finger and thus having reduced DNA binding, failed to substitute for H-NS. Mouse protein Btcd was shown to repress the bgl operon at the level of transcription initiation and to bind preferentially to a curved DNA fragment encompassing the bgl promoter. These effects of Btcd on bacterial gene transcription can be accounted for by the binding of Btcd or H-NS to a curved DNA sequence near a promoter. A few mammalian proteins have been shown to substitute for their Escherichia prototypes involved in DNA and RNA transactions. The efficiency of Btcd protein in substituting for H-NS in Escherichia suggests its possible involvement in regulating gene expression in mouse cells.

MeSH Terms
Animals Bacterial Outer Membrane Proteins/metabolism Bacterial Proteins Base Sequence DNA, Circular/metabolism DNA-Binding Proteins/metabolism Electrophoresis, Gel, Two-Dimensional Escherichia coli/genetics,metabolism Flagella/genetics Gene Expression Regulation, Bacterial Mice Molecular Sequence Data Operon Promoter Regions, Genetic Transcription, Genetic beta-Glucosidase/metabolism
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA, Circular DNA-Binding Proteins H-NS protein, bacteria beta-Glucosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Timchenko T
Groupe d'Etude 'Mutagénèse et Cancérogenèse', Institut Curie, Batiment110, Centre Universitaire, F-91405 Orsay, France.
Bailone A
Devoret R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-08-01
Pages
3986-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452118
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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