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PMID: 1903834 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance.

Molecular & general genetics : MGG ·Vol. 226 ·No. 3 ·1991-05-00 ·Pages 367-76

Tanaka K, Muramatsu S, Yamada H, Mizuno T

Abstract

In addition to the set of curved DNA segments isolated previously from Escherichia coli, another set of curved DNA segments has now been isolated. To gain an insight into the functional significance of these curved DNA sequences, systematic analyses were carried out, which included not only mapping of the precise locations of the segments on the E. coli chromosome but also clarification of the gene organization in the chromosomal regions surrounding the curved DNA sequences. It was demonstrated that most of the curved DNA sequences, which have been characterized so far, appear to be located immediately upstream of the coding sequences of adjacent genes. It was also demonstrated that an E. coli histone-like protein, named H-NS (or H1a), exhibits a strong affinity for naturally occurring curved DNA sequences in regions upstream promoters.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence Blotting, Southern Chromosomes, Bacterial DNA/genetics DNA, Bacterial/genetics DNA-Binding Proteins/metabolism Electrophoresis, Gel, Two-Dimensional Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Nucleic Acid Conformation Plasmids Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins H-NS protein, bacteria DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tanaka K
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
Muramatsu S
Yamada H
Mizuno T
References (47)
47 references, click to expand
  1. Rotational orientation of upstream curved DNA affects promoter function in Bacillus subtilis.
    J Biol Chem. 1989 Jun 25;264(18):10451-6 PMID: 2543669
  2. Partial characterization of a temperature-sensitive mutation affecting acetyl coenzyme A carboxylase in Escherichia coli K-12.
    J Bacteriol. 1976 Jun;126(3):1351-4 PMID: 7549
  3. cur-1, a mutation affecting a phenotype of sup+ strains of Escherichia coli.
    Mol Gen Genet. 1980;180(2):425-8 PMID: 7007815
  4. Nucleotide sequence of the region encompassing the int gene of a cryptic prophage and the dna Y gene flanked by a curved DNA sequence of Escherichia coli K12.
    Mol Gen Genet. 1990 Jan;220(2):325-8 PMID: 2183007
  5. Identification, cloning, nucleotide sequence and chromosomal map location of hns, the structural gene for Escherichia coli DNA-binding protein H-NS.
    Mol Gen Genet. 1988 May;212(2):199-202 PMID: 2841565
  6. Histone-like proteins and bacterial chromosome structure.
    J Biol Chem. 1988 Sep 15;263(26):12793-6 PMID: 3047111
  7. Sequence distributions associated with DNA curvature are found upstream of strong E. coli promoters.
    Nucleic Acids Res. 1987 Jan 26;15(2):785-96 PMID: 3547329
  8. Sequence homology between two membrane transport ATPases, the Kdp-ATPase of Escherichia coli and the Ca2+-ATPase of sarcoplasmic reticulum.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4746-50 PMID: 6146979
  9. A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli.
    Cell. 1988 Feb 26;52(4):569-84 PMID: 2830029
  10. DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS.
    Biochem J. 1984 Dec 15;224(3):799-815 PMID: 6395859
  11. Transcriptional silencing and thermoregulation of gene expression in Escherichia coli.
    Nature. 1990 Apr 12;344(6267):682-5 PMID: 1691451
  12. Linkage map of Escherichia coli K-12, edition 8.
    Microbiol Rev. 1990 Jun;54(2):130-97 PMID: 2194094
  13. Cryptic operon for beta-glucoside metabolism in Escherichia coli K12: genetic evidence for a regulatory protein.
    Genetics. 1981 Jan;97(1):11-25 PMID: 6266910
  14. Nucleotide sequence of the region encompassing the glpKF operon and its upstream region containing a bent DNA sequence of Escherichia coli.
    Nucleic Acids Res. 1989 Jun 12;17(11):4378 PMID: 2544860
  15. Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence.
    J Bacteriol. 1989 May;171(5):2879-81 PMID: 2651420
  16. Synthetic curved DNA sequences can act as transcriptional activators in Escherichia coli.
    EMBO J. 1989 Dec 20;8(13):4289-96 PMID: 2512122
  17. Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase.
    Eur J Biochem. 1985 Jun 3;149(2):363-73 PMID: 3158524
  18. The E. coli dnaY gene encodes an arginine transfer RNA.
    Cell. 1986 May 9;45(3):453-9 PMID: 3516415
  19. DNA structure. Bent molecules--how and why?
    Nature. 1986 Apr 10-16;320(6062):487-8 PMID: 3960132
  20. Escherichia coli glycerol kinase. Cloning and sequencing of the glpK gene and the primary structure of the enzyme.
    J Biol Chem. 1988 Jan 5;263(1):135-9 PMID: 2826434
  21. Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.
    J Bacteriol. 1989 Apr;171(4):1923-31 PMID: 2649479
  22. The DNA binding domain and bending angle of E. coli CAP protein.
    Cell. 1986 Dec 26;47(6):995-1005 PMID: 3536129
  23. Insertion sequence IS5 contains a sharply curved DNA structure at its terminus.
    Mol Gen Genet. 1988 Nov;214(3):433-8 PMID: 2851094
  24. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  25. Sequence specificity of curved DNA.
    FEBS Lett. 1986 Jan 20;195(1-2):53-6 PMID: 3943623
  26. Tandem promoters direct E. coli ribosomal RNA synthesis.
    Cell. 1979 May;17(1):225-34 PMID: 110460
  27. Bending and supercoiling of DNA at the attachment site of bacteriophage lambda.
    Trends Biochem Sci. 1990 Jun;15(6):222-7 PMID: 2166364
  28. DNA supercoiling and environmental regulation of virulence gene expression in Shigella flexneri.
    Nature. 1990 Apr 19;344(6268):789-92 PMID: 2184366
  29. An Escherichia coli protein that preferentially binds to sharply curved DNA.
    J Biochem. 1990 Sep;108(3):420-5 PMID: 2126011
  30. DNA bending at adenine . thymine tracts.
    Nature. 1986 Apr 10-16;320(6062):501-6 PMID: 3960133
  31. Rigorous pattern-recognition methods for DNA sequences. Analysis of promoter sequences from Escherichia coli.
    J Mol Biol. 1985 Nov 5;186(1):117-28 PMID: 3908689
  32. Random cloning of bent DNA segments from Escherichia coli chromosome and primary characterization of their structures.
    Nucleic Acids Res. 1987 Sep 11;15(17):6827-41 PMID: 3309887
  33. Requirement for an upstream element for optimal transcription of a bacterial tRNA gene.
    Nature. 1983 Sep 15-21;305(5931):248-50 PMID: 6350894
  34. Effect of polyadenine-containing curved DNA on promoter utilization in Bacillus subtilis.
    J Biol Chem. 1988 Aug 25;263(24):11743-9 PMID: 3136165
  35. H1a, an E. coli DNA-binding protein which accumulates in stationary phase, strongly compacts DNA in vitro.
    Nucleic Acids Res. 1984 Jul 11;12(13):5321-40 PMID: 6379600
  36. DNA structure. Curves with a function.
    Nature. 1989 Sep 21;341(6239):184-5 PMID: 2779666
  37. The molecular cloning of the gene encoding the Escherichia coli 75-kDa helicase and the determination of its nucleotide sequence and gentic map position.
    J Biol Chem. 1989 May 15;264(14):8297-303 PMID: 2542273
  38. Identification of two new promoters probably involved in the transcription of a ribosomal RNA gene of Escherichia coli.
    Biochim Biophys Acta. 1983 Mar 10;739(2):173-80 PMID: 6297582
  39. Intrinsically bent DNA.
    J Biol Chem. 1990 May 5;265(13):7093-6 PMID: 2185240
  40. Metastable regulation of type 1 piliation in Escherichia coli and isolation and characterization of a phenotypically stable mutant.
    J Bacteriol. 1986 Oct;168(1):179-85 PMID: 3019997
  41. Nucleotide sequence of the fabE gene and flanking regions containing a bent DNA sequence of Escherichia coli.
    Nucleic Acids Res. 1989 May 25;17(10):3982 PMID: 2660106
  42. Nucleotide sequence of the Escherichia coli mutH gene.
    Nucleic Acids Res. 1987 Apr 10;15(7):3073-84 PMID: 3031619
  43. The locus of sequence-directed and protein-induced DNA bending.
    Nature. 1984 Apr 5-11;308(5959):509-13 PMID: 6323997
  44. Conformational change in the DNA associated with an unusual promoter mutation in a tRNA operon of Salmonella.
    Cell. 1984 Dec;39(3 Pt 2):643-52 PMID: 6096016
  45. Physico-chemical properties of a DNA binding protein: Escherichia coli factor H1.
    Eur J Biochem. 1977 Nov 15;81(1):79-90 PMID: 338303
  46. Sequence-directed curvature of DNA.
    Nature. 1986 May 22-28;321(6068):449-50 PMID: 3713816
  47. DNA determinants of rRNA synthesis in E. coli: growth rate dependent regulation, feedback inhibition, upstream activation, antitermination.
    Cell. 1986 Jan 17;44(1):197-205 PMID: 2416474
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00260648
Published
1991-05-00
Pages
367-76
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X63978, X63979, X63980, X63981, X63982, X63983, X63984, X63985, X63986, X63987
External Links
PubMed source DOI: 10.1007/BF00260648
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