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PMID: 3309887 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Random cloning of bent DNA segments from Escherichia coli chromosome and primary characterization of their structures.

Nucleic acids research ·Vol. 15 ·No. 17 ·1987-09-11 ·Pages 6827-41

Mizuno T

Abstract

A simple method for the selective detection of DNA segments containing a sequence-directed static bend was developed. Two-dimensional polyacrylamide gel electrophoresis performed at two different temperatures (60 degrees C and 10 degrees C) can effectively separate a bent DNA from a mixture of normal DNA. Using this method, a bank of plasmids carrying bent DNA inserts from the E. coli total chromosome was constructed. The primary characterization of a set of bent DNA segments randomly cloned from E. coli was presented.

MeSH Terms
Base Sequence Chromosomes, Bacterial/physiology Cloning, Molecular DNA, Bacterial/genetics,isolation & purification Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Nucleic Acid Conformation Plasmids
Chemicals
DNA, Bacterial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Mizuno T
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.17.6827
Published
1987-09-11
Pages
6827-41
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306178
Subset
IM
Databases
GENBANK
X05960, X05961, X05962, X05963, X05964, X05965, X05966, X05967, X05968
External Links
PubMed source DOI: 10.1093/nar/15.17.6827 PMC Full Text View journal details
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