Abstract
The nucleotide sequence of a 2.5 kb region encompassing a curved DNA segment (BENT-9) randomly cloned from the total Escherichia coli chromosome was determined. This region was found to contain the dna Y gene encoding a transfer RNA. The curved DNA structure was demonstrated to be located just upstream of the dna Y promoter. The results of sequencing further revealed that the int gene of a cryptic prophage, qsr', which has been shown to be present in the E. coli genome, is located next to the dna Y gene.
MeSH Terms
Amino Acid Sequence
Base Sequence
Coliphages/genetics
DNA, Bacterial/genetics
Escherichia coli/genetics
Genes, Bacterial
Genes, Viral
Molecular Sequence Data
Promoter Regions, Genetic
RNA, Transfer/genetics
Restriction Mapping
Chemicals
DNA, Bacterial
RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Muramatsu S
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
Mizuno T
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