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PMID: 8670893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The hybrid histidine kinase DokA is part of the osmotic response system of Dictyostelium.

The EMBO journal ·Vol. 15 ·No. 15 ·1996-08-01 ·Pages 3880-9

Schuster SC, Noegel AA, Oehme F, Gerisch G, Simon MI

Abstract

We have used PCR to identify a Dictyostelium homolog of the bacterial two-component system. The gene dokA codes for a member of the hybrid histidine kinase family which is defined by the presence of conserved amino acid sequence motifs corresponding to an N-terminal receptor domain, a central kinase and a C-terminal response regulator moiety. Potential function of the regulator domain was demonstrated by phosphorylation in vitro. dokA mutants are deficient in the osmoregulatory pathway, resulting in premature cell death under high osmotic stress. Under less stringent osmotic conditions, cells grow at a normal rate, but development at the multicellular stage is altered. dokA is a member of a family of histidine kinase-like genes that play regulatory roles in eukaryotic cell function.

MeSH Terms
Amino Acid Sequence Animals Cloning, Molecular Conserved Sequence Dictyostelium/enzymology,genetics,physiology Histidine Kinase Molecular Sequence Data Morphogenesis Osmolar Concentration Polymerase Chain Reaction Protein Kinases/metabolism RNA, Messenger/metabolism Signal Transduction Up-Regulation
Chemicals
RNA, Messenger Protein Kinases Histidine Kinase DokA protein, Dictyostelium discoideum
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schuster S C
Abteilung fur Membranebiochemie, Max-Planck-Institut für Biochemie, 82152 Martinsried, Germany.
Noegel A A
Oehme F
Gerisch G
Simon M I
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-08-01
Pages
3880-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452086
Subset
IM
Databases
GENBANK
X96869
External Links
PubMed source PMC Full Text View journal details
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