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PMID: 8508771 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activation of the prespore and spore cell pathway of Dictyostelium differentiation by cAMP-dependent protein kinase and evidence for its upstream regulation by ammonia.

The EMBO journal ·Vol. 12 ·No. 6 ·1993-06-00 ·Pages 2459-66

Hopper NA, Harwood AJ, Bouzid S, Véron M, Williams JG

Abstract

Expression of a dominant inhibitor of the Dictyostelium cAMP-dependent protein kinase in prespore cells blocks their differentiation into spore cells. The resultant structures comprise a normal stalk supporting a bolus of cells that fail to express a sporulation-specific gene and that show greatly reduced levels of expression of several prespore-specific genes. The latter result suggests that in addition to activating spore formation, the cAMP-dependent protein kinase may play a role in initial prespore cell differentiation. Development of the strain expressing the dominant inhibitor is hypersensitive to the inhibitory effects of ammonia, the molecule that is believed to repress entry into culmination during normal development. This result supports a model whereby a decrease in ambient ammonia concentration at culmination acts to elevate intracellular cAMP and hence induce terminal differentiation.

Related Genes
Dd31 psA
MeSH Terms
Ammonia/metabolism Animals Dictyostelium/drug effects,genetics,physiology Gene Expression Genes, Protozoan Mutation Protein Kinases/metabolism Protozoan Proteins/genetics,metabolism Spores
Chemicals
Protozoan Proteins Ammonia Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hopper N A
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
Harwood A J
Bouzid S
Véron M
Williams J G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-06-00
Pages
2459-66
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413481
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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