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PMID: 8642653 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A large region within the Rous sarcoma virus matrix protein is dispensable for budding and infectivity.

Journal of virology ·Vol. 70 ·No. 4 ·1996-04-00 ·Pages 2269-76

Nelle TD, Wills JW

Abstract

All retroviruses have a layer of matrix protein (MA) situated directly beneath the lipid of their envelope. This protein is initially expressed as the amino-terminal sequence of the Gag polyprotein, where it plays an important role in binding Gag to the plasma membrane during the early steps of the budding process. Others have suggested that MA may provide additional functions during virion assembly, including the selective incorporation of viral glycoproteins and the RNA genome into the emerging virion. To further study the role of the Rous sarcoma virus MA sequence in the viral replication cycle, we have pursued an extensive deletion analysis. Surprisingly, the entire second half of MA (residues 87 to 155) and part of the neighboring p2 sequence were found to be dispensable not only for budding but also for infectivity in avian cells. Thus, all of the functions associated with the Rous sarcoma virus MA sequence must be contained within its first half.

MeSH Terms
Amino Acid Sequence Avian Sarcoma Viruses/genetics,metabolism,pathogenicity Base Sequence Cell Line DNA, Viral Genes, gag Molecular Sequence Data Sequence Deletion Viral Matrix Proteins/genetics,physiology Virus Replication
Chemicals
DNA, Viral Viral Matrix Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nelle T D
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA.
Wills J W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.4.2269-2276.1996
Published
1996-04-00
Pages
2269-76
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190068
Subset
IM
Grants
NCI NIH HHS · CA60395 · United States
NCI NIH HHS · R01 CA47482 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.4.2269-2276.1996 PMC Full Text View journal details
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