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PMID: 6090691 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fine-structure analyses of lipid-protein and protein-protein interactions of gag protein p19 of the avian sarcoma and leukemia viruses by cyanogen bromide mapping.

Journal of virology ·Vol. 52 ·No. 1 ·1984-10-00 ·Pages 145-53

Pepinsky RB, Vogt VM

Abstract

In avian sarcoma and leukemia viruses, the gag protein p19 functions structurally as a matrix protein, connecting internal components with the viral envelope. We have used a combination of in situ cross-linking and peptide mapping to localize within p19 the regions responsible for two major interactions in this complex, p19 with lipid and p19 with p19. Lipid-protein cross-links were localized near the amino terminus within the first 35 amino acids of the polypeptide. Homotypic protein-protein disulfide bridges were found to originate from near the carboxy terminus of p19, from cysteine residues at amino acids 111 and 153. These results suggest that p19 is divided into domains with distinct functions. The peptide maps constructed for p19, and for the related proteins p23 in avian sarcoma and leukemia viruses and p19 beta in recombinant avian sarcoma viruses, should serve as useful tools for other types of studies involving these proteins.

MeSH Terms
Amino Acids/analysis Animals Antigens, Viral/analysis Avian Leukosis Virus/analysis Avian Myeloblastosis Virus/analysis Avian Sarcoma Viruses/analysis Chick Embryo Cyanogen Bromide Disulfides/analysis Electrophoresis, Polyacrylamide Gel Fibroblasts Gene Products, gag Lipoproteins/analysis Peptide Fragments/analysis Viral Proteins/analysis
Chemicals
Amino Acids Antigens, Viral Disulfides Gene Products, gag Lipoproteins Peptide Fragments Viral Proteins Cyanogen Bromide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pepinsky R B
Vogt V M
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39 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.52.1.145-153.1984
Published
1984-10-00
Pages
145-53
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254500
Subset
IM
Grants
NCI NIH HHS · CA-20081 · United States
External Links
PubMed source DOI: 10.1128/JVI.52.1.145-153.1984 PMC Full Text View journal details
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