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PMID: 8628674 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Afut1, a retrotransposon-like element from Aspergillus fumigatus.

Nucleic acids research ·Vol. 24 ·No. 8 ·1996-04-15 ·Pages 1428-34

Neuveglise C, Sarfati J, Latge JP, Paris S

Abstract

A repeated DNA sequence used for epidemiological studies of the human opportunistic pathogen Aspergillus fumigatus has been characterized. It is a retroelement of 6914 bp in length, bounded by long terminal repeats of 282 bp, with sequence and features characteristic of retroviruses and retrotransposons. A 5 bp duplication site was found at its borders. This element, designated Afut1, encodes amino acid sequences homologous to the reverse transcriptase, RNase H and endonuclease encoded by the pol genes of retroelements. Comparison of the peptidic sequences with other putative polypeptides of fungal LTR retrotransposons showed that Afut1 is a member of the gypsy group. This is the first report of a transposable element in A.fumigatus. Afut1 is a defective element: the putative coding domains contain multiple stop codons due exclusively to transitions from C:G to T:A.

MeSH Terms
Amino Acid Sequence Aspergillus fumigatus/genetics Base Sequence DNA, Fungal Fungal Proteins/chemistry,genetics Gene Dosage Humans Molecular Sequence Data Repetitive Sequences, Nucleic Acid Retroelements Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
Chemicals
Afut1 protein, Aspergillus fumigatus DNA, Fungal Fungal Proteins Retroelements
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Neuveglise C
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
Sarfati J
Latge J P
Paris S
References (36)
36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.8.1428
Published
1996-04-15
Pages
1428-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC145799
Subset
IM
Databases
GENBANK
L76085, L76086
External Links
PubMed source DOI: 10.1093/nar/24.8.1428 PMC Full Text View journal details
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