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PMID: 8616222 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The GATA-binding protein CGF-1 is closely related to GT-1.

Plant molecular biology ·Vol. 29 ·No. 6 ·1995-12-00 ·Pages 1253-66

Teakle GR, Kay SA

Abstract

Many light-regulated genes contain a conserved GATA motif in their 5'-upstream region. We have characterized in detail the GATA-binding factor, CGF-1, which bonds within a 73 bp TATA-proximal light/circadian regulatory element in the Arabidopsis cab2 promoter and to two more sites farther upstream. CGF-1 was found to be distinct from other metal-dependent GATA-binding factors, but to have the same sequence requirements for binding and similar physical and chemical properties as GT-1, a factor required for light regulation of the tobacco rbcS-3A gene. CGF-1 was found to be constitutively present in extracts and was shown to be immunologically related to GT-1. The close similarity between CGF-1 and GT-1 suggests that a GT-1-like factor is involved in the phytochrome/circadian regulation of the cab2 gene. CGF-1 and GT-1 were also found to have similar sequence specificities to another constitutively-regulated GATA factor, IBF-2b, which binds the I box region of the tomato nitrate reductase gene. Of three complexes detected using an IBF-2b-specific probe, only one was identical to CGF-1/GT-1. The other two were similar to IBF-2b, demonstrating that CGF-1/GT-1, although very similar, are actually distinct from IBF-2b. These data indicate that more than one factor can bind to the same short sequence and may indicate how constitutively present factors like GT-1 can play a role in light regulation.

MeSH Terms
Arabidopsis/genetics,metabolism Base Sequence Circadian Rhythm Conserved Sequence DNA-Binding Proteins/biosynthesis,metabolism Gene Expression Regulation, Plant Genes, Plant Light Molecular Sequence Data Nuclear Proteins/biosynthesis,metabolism Plants, Toxic Promoter Regions, Genetic Ribulose-Bisphosphate Carboxylase/genetics TATA Box Tobacco/enzymology,genetics Transcription Factors/biosynthesis,metabolism
Chemicals
DNA-Binding Proteins GT-1 transcription factor Nuclear Proteins Transcription Factors Ribulose-Bisphosphate Carboxylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Teakle G R
Department of Biology, University of Virginia, Charlottesville 22903, USA.
Kay S A
References (47)
47 references, click to expand
  1. Localization and conditional redundancy of regulatory elements in rbcS-3A, a pea gene encoding the small subunit of ribulose-bisphosphate carboxylase.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4662-6 PMID: 3387433
  2. An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7089-93 PMID: 2902624
  3. Molecular dissection of GT-1 from Arabidopsis.
    Plant Cell. 1994 Dec;6(12):1805-13 PMID: 7866025
  4. Isolation and Characterization of a Ferredoxin Gene from Arabidopsis thaliana.
    Plant Physiol. 1990 Jun;93(2):572-7 PMID: 16667505
  5. DNA-binding specificity of GATA family transcription factors.
    Mol Cell Biol. 1993 Jul;13(7):3999-4010 PMID: 8321207
  6. Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements.
    Genes Dev. 1987 May;1(3):247-55 PMID: 2890552
  7. Discrimination of phytochrome dependent light inducible from non-light inducible plant genes. Prediction of a common light-responsive element (LRE) in phytochrome dependent light inducible plant genes.
    Nucleic Acids Res. 1987 Dec 10;15(23):9957-73 PMID: 3697087
  8. Novel cis-acting elements in Petunia Cab gene promoters.
    Mol Gen Genet. 1989 Jan;215(2):337-44 PMID: 2651885
  9. Mutation of either G box or I box sequences profoundly affects expression from the Arabidopsis rbcS-1A promoter.
    EMBO J. 1990 Jun;9(6):1717-26 PMID: 2347304
  10. Deletion analysis of a phytochrome-regulated monocot rbcS promoter in a transient assay system.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2683-6 PMID: 2011579
  11. Constitutive, light-responsive and circadian clock-responsive factors compete for the different l box elements in plant light-regulated promoters.
    Plant J. 1993 Oct;4(4):611-9 PMID: 8252065
  12. DNA binding factor GT-2 from Arabidopsis.
    Plant Mol Biol. 1993 Oct;23(2):337-48 PMID: 8219069
  13. Light-regulated modification and nuclear translocation of cytosolic G-box binding factors in parsley.
    Plant Cell. 1994 Apr;6(4):545-59 PMID: 8205004
  14. A tobacco DNA binding protein that interacts with a light-responsive box II element.
    Plant Cell. 1992 Jul;4(7):831-8 PMID: 1392597
  15. Cloning and analysis of the tomato nitrate reductase-encoding gene: protein domain structure and amino acid homologies in higher plants.
    Gene. 1989 Dec 28;85(2):371-80 PMID: 2628174
  16. Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia.
    EMBO J. 1988 Jul;7(7):1929-36 PMID: 2901343
  17. Functional dissection of circadian clock- and phytochrome-regulated transcription of the Arabidopsis CAB2 gene.
    Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1500-4 PMID: 7878008
  18. Characterization of a gene encoding a DNA binding protein with specificity for a light-responsive element.
    Plant Cell. 1992 Jul;4(7):839-49 PMID: 1392598
  19. Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene.
    EMBO J. 1987 Sep;6(9):2543-9 PMID: 3678200
  20. Minimal sequence requirements for the regulated expression of rbcS-3A from Pisum sativum in transgenic tobacco plants.
    Photochem Photobiol. 1990 Jul;52(1):43-50 PMID: 2399285
  21. Molecular characterization of two clusters of genes encoding the Type I CAB polypeptides of PSII in Nicotiana plumbaginifolia.
    Plant Mol Biol. 1987 Mar;10(2):117-26 PMID: 24277497
  22. Two 10-bp regions are critical for phytochrome regulation of a Lemna gibba Lhcb gene promoter.
    Plant Cell. 1994 Aug;6(8):1123-34 PMID: 7919982
  23. Spacing between GT-1 binding sites within a light-responsive element is critical for transcriptional activity.
    Plant Cell. 1990 May;2(5):447-55 PMID: 2152170
  24. A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter.
    Plant Cell. 1990 Aug;2(8):805-14 PMID: 2152129
  25. Photoregulated gene expression may involve ubiquitous DNA binding proteins.
    EMBO J. 1990 Nov;9(11):3415-27 PMID: 2209551
  26. NMR structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1.
    Science. 1993 Jul 23;261(5120):438-46 PMID: 8332909
  27. A single point mutation is the cause of the Greek form of hereditary persistence of fetal haemoglobin.
    Nature. 1992 Aug 6;358(6386):499-502 PMID: 1379347
  28. A trans-acting factor that binds to a GT-motif in a phytochrome gene promoter.
    Science. 1990 Dec 7;250(4986):1397-9 PMID: 2255908
  29. A tobacco cDNA clone encoding a GATA-1 zinc finger protein homologous to regulators of nitrogen metabolism in fungi.
    Mol Gen Genet. 1993 Sep;240(3):365-73 PMID: 8413186
  30. Circadian clock- and phytochrome-regulated transcription is conferred by a 78 bp cis-acting domain of the Arabidopsis CAB2 promoter.
    Plant J. 1994 Oct;6(4):457-70 PMID: 7987408
  31. A metal-dependent DNA-binding protein interacts with a constitutive element of a light-responsive promoter.
    Plant Cell. 1990 Sep;2(9):857-66 PMID: 2152132
  32. Phytochrome regulated gene expression.
    Semin Cell Biol. 1994 Oct;5(5):335-46 PMID: 7881073
  33. Determination of steady-state mRNA levels of individual chlorophyll a/b binding protein genes of the tomato cab gene family.
    Mol Gen Genet. 1991 Dec;230(3):413-22 PMID: 1766438
  34. Localization of a phytochrome-responsive element within the upstream region of pea rbcS-3A.
    Mol Cell Biol. 1990 Oct;10(10):5565-8 PMID: 2398902
  35. nit-2, the major nitrogen regulatory gene of Neurospora crassa, encodes a protein with a putative zinc finger DNA-binding domain.
    Mol Cell Biol. 1990 Mar;10(3):1056-65 PMID: 2137552
  36. Organization and expression of the genes encoding ribulose-1,5-bisphosphate carboxylase in higher plants.
    Photosynth Res. 1988 Apr;16(1-2):117-39 PMID: 24430995
  37. GT-1 binding site confers light responsive expression in transgenic tobacco.
    Science. 1990 Apr 27;248(4954):471-4 PMID: 2330508
  38. Binding sites for two novel phosphoproteins, 3AF5 and 3AF3, are required for rbcS-3A expression.
    Plant Cell. 1992 Apr;4(4):473-83 PMID: 1498605
  39. Maize rbcS promoter activity depends on sequence elements not found in dicot rbcS promoters.
    Plant Cell. 1991 Sep;3(9):997-1012 PMID: 1822995
  40. The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger.
    EMBO J. 1990 May;9(5):1355-64 PMID: 1970293
  41. Molecular light switches for plant genes.
    Plant Cell. 1990 May;2(5):369-78 PMID: 2152164
  42. GT-2: a transcription factor with twin autonomous DNA-binding domains of closely related but different target sequence specificity.
    EMBO J. 1992 Nov;11(11):4131-44 PMID: 1396594
  43. Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins.
    EMBO J. 1992 Apr;11(4):1261-73 PMID: 1373374
  44. The two zinc finger-like domains of GATA-1 have different DNA binding specificities.
    EMBO J. 1993 Dec 15;12(13):4993-5005 PMID: 8262042
  45. ASF-2: a factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters.
    Plant Cell. 1989 Dec;1(12):1147-56 PMID: 2535536
  46. Binding site requirements for pea nuclear protein factor GT-1 correlate with sequences required for light-dependent transcriptional activation of the rbcS-3A gene.
    EMBO J. 1988 Dec 20;7(13):4035-44 PMID: 3243271
  47. Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness.
    EMBO J. 1991 Jul;10(7):1777-86 PMID: 2050115
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00020466
Published
1995-12-00
Pages
1253-66
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00020466 View journal details
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