Home LiteratureArticle Details
PMID: 7919982 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two 10-bp regions are critical for phytochrome regulation of a Lemna gibba Lhcb gene promoter.

The Plant cell ·Vol. 6 ·No. 8 ·1994-08-00 ·Pages 1123-34

Kehoe DM, Degenhardt J, Winicov I, Tobin EM

Abstract

Two small regions of the promoter of an Lhcb gene encoding a light-harvesting chlorophyll a/b protein were identified as essential in conferring phytochrome responsiveness by using a transient expression assay. Initially, 5' deletion analysis of cabAB19, an Lhcb2 gene of Lemna, showed that sequences within the region from -174 to -104 relative to the start of transcription were necessary for phytochrome regulation. Internal deletion and substitution mutants were used to demonstrate that no additional phytochrome-responsive regions exist between -1600 and -174 in this promoter. A 171-bp fragment of the promoter extending from -239 to -69 was sufficient to impart phytochrome responsiveness to a minimal ubiquitin promoter that was not itself regulated by light. Specific binding of Lemna proteins to the region necessary for phytochrome responsiveness was demonstrated using in vitro polyacrylamide gel mobility shift assays and 1,10-phenanthroline copper ion footprinting. Further analysis of the region from -174 to -104 demonstrated that mutations in two separate 10-bp sequences, from -134 to -125 and from -114 to -105, could abolish phytochrome responsiveness; thus, there are two unique regions that are necessary for phytochrome regulation of this gene. One of these regions contains a CCAAT motif and the other a GATA motif. These motifs are conserved in the promoters of many Lhcb genes and may be important elements in the phytochrome responsiveness of this gene family.

MeSH Terms
Base Sequence DNA Mutational Analysis Gene Expression Regulation, Plant/radiation effects Genes, Reporter Light Light-Harvesting Protein Complexes Luciferases/biosynthesis,genetics Molecular Sequence Data Photosynthetic Reaction Center Complex Proteins/biosynthesis,genetics Phytochrome/metabolism Plant Proteins/metabolism Plants/genetics Promoter Regions, Genetic/genetics Protein Binding Sequence Deletion Sequence Homology, Nucleic Acid Signal Transduction Structure-Activity Relationship Transformation, Genetic Ubiquitins/genetics
Chemicals
Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Plant Proteins Ubiquitins Phytochrome Luciferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kehoe D M
Department of Biology, University of California at Los Angeles 90024-1606.
Degenhardt J
Winicov I
Tobin E M
References (45)
45 references, click to expand
  1. A negatively acting DNA sequence element mediates phytochrome-directed repression of phyA gene transcription.
    EMBO J. 1991 Oct;10(10):3015-24 PMID: 1915276
  2. Photosynthesis-associated gene families: differences in response to tissue-specific and environmental factors.
    Science. 1986 Jul 4;233(4759):34-8 PMID: 17812887
  3. Structure and expression of a pea nuclear gene encoding a chlorophyll a/b-binding polypeptide.
    Proc Natl Acad Sci U S A. 1984 May;81(10):2960-4 PMID: 16593461
  4. Discrimination of phytochrome dependent light inducible from non-light inducible plant genes. Prediction of a common light-responsive element (LRE) in phytochrome dependent light inducible plant genes.
    Nucleic Acids Res. 1987 Dec 10;15(23):9957-73 PMID: 3697087
  5. Novel cis-acting elements in Petunia Cab gene promoters.
    Mol Gen Genet. 1989 Jan;215(2):337-44 PMID: 2651885
  6. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Methods Enzymol. 1987;154:367-82 PMID: 3323813
  7. Eukaryotic transcriptional regulatory proteins.
    Annu Rev Biochem. 1989;58:799-839 PMID: 2673023
  8. Sequence specificity of the deoxyribonuclease activity of 1,10-phenanthroline-copper ion.
    Biochemistry. 1990 Feb 27;29(8):2116-21 PMID: 2328244
  9. Regulation of nuclear genes encoding chloroplast proteins in transgenic plants.
    Dev Genet. 1990;11(3):197-204 PMID: 2279355
  10. Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia.
    EMBO J. 1988 Jul;7(7):1929-36 PMID: 2901343
  11. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation.
    Plant Mol Biol. 1992 Feb;18(4):675-89 PMID: 1313711
  12. Demonstration of transcriptional regulation of specific genes by phytochrome action.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):1112-6 PMID: 16593420
  13. A novel circadian phenotype based on firefly luciferase expression in transgenic plants.
    Plant Cell. 1992 Sep;4(9):1075-87 PMID: 1392609
  14. Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene.
    EMBO J. 1987 Sep;6(9):2543-9 PMID: 3678200
  15. Fresh view of light signal transduction in plants.
    Cell. 1994 Feb 11;76(3):423-6 PMID: 8313465
  16. Footprinting DNA-protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coli RNA polymerase-lac promoter complexes.
    Biochemistry. 1987 Nov 17;26(23):7234-8 PMID: 3322397
  17. Photoregulation of a phytochrome gene promoter from oat transferred into rice by particle bombardment.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9692-6 PMID: 2602370
  18. Identification of upstream regulatory elements involved in the developmental expression of the Arabidopsis thaliana cab1 gene.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):8017-21 PMID: 3054877
  19. Spacing between GT-1 binding sites within a light-responsive element is critical for transcriptional activity.
    Plant Cell. 1990 May;2(5):447-55 PMID: 2152170
  20. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  21. White Light Effects on the mRNA for the Light-Harvesting Chlorophyll a/b-Protein in Lemna gibba L. G-3.
    Plant Physiol. 1981 Jun;67(6):1078-83 PMID: 16661813
  22. Nuclease activity of 1,10-phenanthroline-copper in study of protein-DNA interactions.
    Methods Enzymol. 1991;208:414-33 PMID: 1779842
  23. A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter.
    Plant Cell. 1990 Aug;2(8):805-14 PMID: 2152129
  24. Evolutionary variation of the CCAAT-binding transcription factor NF-Y.
    Nucleic Acids Res. 1992 Mar 11;20(5):1087-91 PMID: 1549471
  25. Garnet: first occurrence in the lunar rocks.
    Science. 1970 Sep 4;169(3949):981-2 PMID: 17838173
  26. New concepts in protein-DNA recognition: sequence-directed DNA bending and flexibility.
    Prog Nucleic Acid Res Mol Biol. 1994;47:195-270 PMID: 8016321
  27. A tobacco cDNA clone encoding a GATA-1 zinc finger protein homologous to regulators of nitrogen metabolism in fungi.
    Mol Gen Genet. 1993 Sep;240(3):365-73 PMID: 8413186
  28. The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses.
    Cell. 1987 Mar 27;48(6):1035-46 PMID: 3030556
  29. Determination of steady-state mRNA levels of individual chlorophyll a/b binding protein genes of the tomato cab gene family.
    Mol Gen Genet. 1991 Dec;230(3):413-22 PMID: 1766438
  30. Luciferase reporter gene cassettes for plant gene expression studies.
    Nucleic Acids Res. 1987 Oct 12;15(19):8115 PMID: 3671076
  31. Light-independent developmental regulation of cab gene expression in Arabidopsis thaliana seedlings.
    Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7791-5 PMID: 1380166
  32. Localization of a phytochrome-responsive element within the upstream region of pea rbcS-3A.
    Mol Cell Biol. 1990 Oct;10(10):5565-8 PMID: 2398902
  33. A chlorophyll a/b-protein encoded by a gene containing an intron with characteristics of a transposable element.
    J Mol Appl Genet. 1985;3(1):45-61 PMID: 2989405
  34. A rice cab gene promoter contains separate cis-acting elements that regulate expression in dicot and monocot plants.
    Plant Cell. 1992 Aug;4(8):971-81 PMID: 1392604
  35. CA-1, a novel phosphoprotein, interacts with the promoter of the cab140 gene in Arabidopsis and is undetectable in det1 mutant seedlings.
    Plant Cell. 1993 Jan;5(1):109-21 PMID: 8439741
  36. Phytochrome: a light-activated molecular switch that regulates plant gene expression.
    Annu Rev Genet. 1991;25:389-409 PMID: 1812812
  37. Nucleotide sequence of Cab-215, a type II gene encoding a photosystem II chlorophyll a/b-binding protein in Pisum.
    Plant Mol Biol. 1991 Jul;17(1):135-9 PMID: 1868214
  38. GT-1 binding site confers light responsive expression in transgenic tobacco.
    Science. 1990 Apr 27;248(4954):471-4 PMID: 2330508
  39. Maize rbcS promoter activity depends on sequence elements not found in dicot rbcS promoters.
    Plant Cell. 1991 Sep;3(9):997-1012 PMID: 1822995
  40. Nucleotide sequence of Cab-8, a new type I gene encoding a chlorophyll a/b-binding protein of LHC II in Pisum.
    Plant Mol Biol. 1991 Sep;17(3):523-6 PMID: 1884005
  41. Developmental and organ-specific changes in promoter DNA-protein interactions in the tomato rbcS gene family.
    Plant Cell. 1991 Dec;3(12):1305-16 PMID: 1840899
  42. Expression of Light-Harvesting Chlorophyll a/b-Protein Genes Is Phytochrome-Regulated in Etiolated Arabidopsis thaliana Seedlings.
    Plant Physiol. 1988 Dec;88(4):1323-31 PMID: 16666462
  43. Analysis of genes negatively regulated by phytochrome action in Lemna gibba and identification of a promoter region required for phytochrome responsiveness.
    Plant Physiol. 1993 Mar;101(3):915-24 PMID: 8310060
  44. ASF-2: a factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters.
    Plant Cell. 1989 Dec;1(12):1147-56 PMID: 2535536
  45. Transcriptional regulation of a gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean tissue is linked to the phytochrome response.
    Mol Cell Biol. 1985 Aug;5(8):1910-7 PMID: 3837851
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.6.8.1123
Published
1994-08-00
Pages
1123-34
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160506
Subset
IM
Grants
NIGMS NIH HHS · GM 23167 · United States
External Links
PubMed source DOI: 10.1105/tpc.6.8.1123 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2