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PMID: 2398902 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Localization of a phytochrome-responsive element within the upstream region of pea rbcS-3A.

Molecular and cellular biology ·Vol. 10 ·No. 10 ·1990-10-00 ·Pages 5565-8

Gilmartin PM, Chua NH

Abstract

The pea rbcS-3A promoter with a 5' deletion to -166 (-166 rbcS-3A) contains two GT-1-binding sites. Mutational analyses demonstrated that a decrease in affinity for GT-1 correlates with reduced promoter activity. Transcription of -166 rbcS-3A in transgenic etiolated seedlings is induced by red light and suppressed by far-red light, indicating that it contains a phytochrome-responsive element.

MeSH Terms
Base Sequence Binding Sites DNA, Recombinant Fabaceae/genetics Genes, Plant Light Molecular Sequence Data Phytochrome/metabolism Plant Proteins/metabolism Plants, Medicinal Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Restriction Mapping Transcription, Genetic
Chemicals
DNA, Recombinant Plant Proteins Phytochrome
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gilmartin P M
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.
Chua N H
References (24)
24 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.10.5565-5568.1990
Published
1990-10-00
Pages
5565-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361276
Subset
IM
Grants
DRS NIH HHS · BRSG507 · United States
NCRR NIH HHS · RR07065 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.10.5565-5568.1990 PMC Full Text View journal details
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