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PMID: 8614621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequences attaching loops of nuclear and mitochondrial DNA to underlying structures in human cells: the role of transcription units.

Nucleic acids research ·Vol. 24 ·No. 7 ·1996-04-01 ·Pages 1212-9

Jackson DA, Bartlett J, Cook PR

Abstract

DNA sequences attaching loops of nuclear and mitochondrial DNA to underlying structures in HeLa cells have been cloned and 106 representative clones sequenced; 10 clones containing random genomic fragments served as controls. As chromatin is prone to rearrangement, care was taken to isolate sequences using 'physiological' conditions that did not create additional attachments. Comparison (by Southern blotting) of the concentration of each cloned sequence in 'total' and 'attached' fractions of DNA showed that most clones did contain attached sequences, but even highly-attached sequences were not attached in all cells in the population. Results demonstrated that 28% of clones were derived from three specific parts of the mitochondrial genome and 22% from different parts of the alu repeat. In addition, 41% of clones contained unique nuclear sequences; these contained no more of the motifs found attached to nuclear scaffolds or matrices (ie SARs or MARs) than would be expected from their base composition. No other attachment motif(s) could be identified by sequence analysis. However, Northern blotting showed that all the mitochondrial clones and 76% of clones containing unique sequences were transcribed; the degree of attachment correlated with transcriptional activity. These results are consistent with transcription being responsible for ever-changing attachments in both nuclei and mitochondria.

MeSH Terms
Base Sequence Cell Nucleus/ultrastructure DNA/metabolism DNA, Mitochondrial/metabolism Deoxyribonucleoproteins/chemistry Gene Expression Regulation, Developmental Genomic Library HeLa Cells Humans Molecular Sequence Data Nuclear Matrix/ultrastructure RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Mitochondrial Deoxyribonucleoproteins RNA, Messenger DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jackson D A
CRC Nuclear Structure and Function Research Group, Sir William Dunn School of Pathology, University of Oxford, UK.
Bartlett J
Cook P R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.7.1212
Published
1996-04-01
Pages
1212-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC145779
Subset
IM
Databases
GENBANK
X89762, X89763, X89764, X89765, X89766, X89767, X89831, X89832, X89833, X89834, X89835, X89836, X89837, X89838, X89839, X89840, X89842, X89843, X89844, X89845, X89846, X89847, X89848, X91537, X91538, X91539, X91540, X91541, X91542, X91543
External Links
PubMed source DOI: 10.1093/nar/24.7.1212 PMC Full Text View journal details
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