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PMID: 8550465 Published · ppublish English Journal Article

Characterization of the Erwinia chrysanthemi osmoprotectant transporter gene ousA.

Journal of bacteriology ·Vol. 178 ·No. 2 ·1996-01-00 ·Pages 447-55

Gouesbet G, Trautwetter A, Bonnassie S, Wu LF, Blanco C

Abstract

Growth of Erwinia chrysanthemi in media of elevated osmolarity can be achieved by the uptake and accumulation of various osmoprotectants. This study deals with the cloning and sequencing of the ousA gene-encoded osmoprotectant uptake system A from E. chrysanthemi 3937. OusA belongs to the superfamily of solute ion cotransporters. This osmotically inducible system allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid and presents strong similarities in nucleotide sequence and protein function with the proline/betaine porter of Escherichia coli encoded by proP. The control of ousA expression is clearly different from that of proP. It is induced by osmotic strength and repressed by osmoprotectants. Its expression in E. coli is controlled by H-NS and is rpoS dependent in the exponential phase but unaffected by the stationary phase.

MeSH Terms
Amino Acid Sequence Amino Acids, Diamino/metabolism Bacterial Proteins/biosynthesis,chemistry,genetics Betaine/metabolism,pharmacology Biological Transport Carrier Proteins/biosynthesis,chemistry,genetics Cloning, Molecular Culture Media DNA-Binding Proteins/genetics,physiology Dickeya chrysanthemi/genetics,metabolism Escherichia coli/genetics,growth & development Escherichia coli Proteins Gene Expression Regulation, Bacterial/drug effects,physiology Genes, Bacterial/genetics Genetic Complementation Test Glucuronidase/biosynthesis,genetics Membrane Transport Proteins Molecular Sequence Data Molecular Weight Nuclear Proteins/genetics,physiology Osmolar Concentration Pipecolic Acids/metabolism Proline/metabolism Recombinant Fusion Proteins/biosynthesis Sequence Alignment Sequence Analysis, DNA Sigma Factor/genetics Symporters
Chemicals
Amino Acids, Diamino Bacterial Proteins Carrier Proteins Culture Media DNA-Binding Proteins Escherichia coli Proteins H-NS protein, bacteria Membrane Transport Proteins Nuclear Proteins OusA protein, Erwinia chrysanthemi Pipecolic Acids ProP protein, E coli Recombinant Fusion Proteins Sigma Factor Symporters sigma factor KatF protein, Bacteria Betaine ectoine Proline Glucuronidase pipecolic acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gouesbet G
Centre National de la Recherche Scientifique (CNRS) URA 256, Département Membranes et Osmorégulation, Université de Rennes I, France.
Trautwetter A
Bonnassie S
Wu L F
Blanco C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.2.447-455.1996
Published
1996-01-00
Pages
447-55
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177677
Subset
IM
Databases
GENBANK
X82267
External Links
PubMed source DOI: 10.1128/jb.178.2.447-455.1996 PMC Full Text View journal details
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