Home LiteratureArticle Details
PMID: 1629159 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Osmoprotection of Escherichia coli by ectoine: uptake and accumulation characteristics.

Journal of bacteriology ·Vol. 174 ·No. 15 ·1992-08-00 ·Pages 5027-35

Jebbar M, Talibart R, Gloux K, Bernard T, Blanco C

Abstract

Ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) is a cyclic amino acid, identified as a compatible solute in moderately halophilic bacteria. Exogenously provided ectoine was found to stimulate growth of Escherichia coli in media of inhibitory osmotic strength. The stimulation was independent of any specific solute, electrolyte or nonelectrolyte. It is accumulated in E. coli cells proportionally to the osmotic strength of the medium, and it is not metabolized. Its osmoprotective ability was as potent as that of glycine betaine. The ProP and ProU systems are both involved in ectoine uptake and accumulation in E. coli. ProP being the main system for ectoine transport. The intracellular ectoine pool is regulated by both influx and efflux systems.

MeSH Terms
Amino Acids, Diamino/pharmacokinetics,pharmacology Biological Transport Carrier Proteins/physiology Escherichia coli/drug effects,metabolism Genes, Bacterial Osmolar Concentration Proline/genetics,metabolism
Chemicals
Amino Acids, Diamino Carrier Proteins ectoine Proline
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jebbar M
Centre National de la Recherche Scientifique URA 256, Laboratoire de Génétique et Physiologie Microbiennes, Rennes, France.
Talibart R
Gloux K
Bernard T
Blanco C
References (38)
38 references, click to expand
  1. Purification and properties of the sn-glycerol 3-phosphate-binding protein of Escherichia coli.
    J Biol Chem. 1979 Nov 10;254(21):10931-5 PMID: 387762
  2. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  3. Microbial water stress.
    Bacteriol Rev. 1976 Dec;40(4):803-46 PMID: 1008746
  4. Prokaryotic osmoregulation: genetics and physiology.
    Annu Rev Microbiol. 1991;45:569-606 PMID: 1741624
  5. Regulation of compatible solute accumulation in Salmonella typhimurium: evidence for a glycine betaine efflux system.
    J Gen Microbiol. 1991 Nov;137(11):2617-25 PMID: 1783908
  6. Characterization of mutations affecting the osmoregulated proU promoter of Escherichia coli and identification of 5' sequences required for high-level expression.
    J Bacteriol. 1991 Jan;173(2):801-9 PMID: 1846150
  7. Identification of an osmotically induced periplasmic glycine betaine-binding protein from Rhizobium meliloti.
    Biochim Biophys Acta. 1991 Jan 30;1061(2):197-205 PMID: 1847827
  8. Escherichia coli accumulates the eukaryotic osmolyte taurine at high osmolarity.
    FEMS Microbiol Lett. 1991 Jun 15;65(2):209-13 PMID: 1884995
  9. Isolation and structure determination of a novel compatible solute from the moderately halophilic purple sulfur bacterium Ectothiorhodospira marismortui.
    Eur J Biochem. 1991 Jun 15;198(3):593-8 PMID: 2050139
  10. Trehalose transport and metabolism in Escherichia coli.
    J Bacteriol. 1990 Jun;172(6):3450-61 PMID: 2160944
  11. 13C NMR study of the interrelation between synthesis and uptake of compatible solutes in two moderately halophilic eubacteria. Bacterium Ba1 and Vibro costicola.
    Arch Biochem Biophys. 1990 Apr;278(1):106-12 PMID: 2321951
  12. Insertion proQ220::Tn5 alters regulation of proline porter II, a transporter of proline and glycine betaine in Escherichia coli.
    J Bacteriol. 1989 Feb;171(2):947-51 PMID: 2536686
  13. In vitro reconstitution of osmoregulated expression of proU of Escherichia coli.
    Proc Natl Acad Sci U S A. 1989 Feb;86(4):1153-7 PMID: 2645575
  14. Osmoregulation in Escherichia coli: complementation analysis and gene-protein relationships in the proU locus.
    J Bacteriol. 1989 Apr;171(4):1915-22 PMID: 2649478
  15. Oxidative pathway of choline to betaine in the soluble fraction prepared from Arthrobacter globiformis.
    J Biochem. 1977 Jul;82(1):157-63 PMID: 197073
  16. Role of amino acids in osmoregulation of non-halophilic bacteria.
    Nature. 1975 Oct 2;257(5525):398-400 PMID: 241020
  17. Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.
    J Bacteriol. 1989 Apr;171(4):1923-31 PMID: 2649479
  18. Physiological and genetic responses of bacteria to osmotic stress.
    Microbiol Rev. 1989 Mar;53(1):121-47 PMID: 2651863
  19. Cloned structural genes for the osmotically regulated binding-protein-dependent glycine betaine transport system (ProU) of Escherichia coli K-12.
    Mol Microbiol. 1988 Mar;2(2):265-79 PMID: 2837616
  20. Proline-hyperproducing strains of Serratia marcescens: enhancement of proline analog-mediated growth inhibition by increasing osmotic stress.
    Appl Environ Microbiol. 1985 Apr;49(4):782-6 PMID: 2860849
  21. Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose.
    Arch Microbiol. 1987 Feb;147(1):1-7 PMID: 2883950
  22. Binding protein dependent transport of glycine betaine and its osmotic regulation in Escherichia coli K12.
    Mol Gen Genet. 1986 Nov;205(2):225-33 PMID: 2949137
  23. Transient accumulation of potassium glutamate and its replacement by trehalose during adaptation of growing cells of Escherichia coli K-12 to elevated sodium chloride concentrations.
    Arch Microbiol. 1988;150(4):348-57 PMID: 3060036
  24. proP-mediated proline transport also plays a role in Escherichia coli osmoregulation.
    J Bacteriol. 1986 Apr;166(1):331-3 PMID: 3082857
  25. Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli.
    J Bacteriol. 1988 Jun;170(6):2841-9 PMID: 3131312
  26. Purification and characterization of a glycine betaine binding protein from Escherichia coli.
    J Biol Chem. 1987 Aug 25;262(24):11841-6 PMID: 3305496
  27. Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli.
    J Bacteriol. 1986 Mar;165(3):849-55 PMID: 3512525
  28. Proline transport and osmotic stress response in Escherichia coli K-12.
    J Bacteriol. 1986 Apr;166(1):253-9 PMID: 3514577
  29. Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassium.
    J Bacteriol. 1986 Nov;168(2):805-14 PMID: 3536861
  30. 1,4,5,6-Tetrahydro-2-methyl-4-pyrimidinecarboxylic acid. A novel cyclic amino acid from halophilic phototrophic bacteria of the genus Ectothiorhodospira.
    Eur J Biochem. 1985 May 15;149(1):135-9 PMID: 3838936
  31. Salmonella typhimurium proP gene encodes a transport system for the osmoprotectant betaine.
    J Bacteriol. 1985 Dec;164(3):1218-23 PMID: 3905767
  32. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.
    J Biol Chem. 1965 Sep;240(9):3685-92 PMID: 4284300
  33. Study of binding protein-ligand interaction by ammonium sulfate-assisted adsorption on cellulose esters filters.
    Biochim Biophys Acta. 1983 Jan 12;742(1):16-24 PMID: 6337632
  34. Glutamine and proline accumulation by Staphylococcus aureus with reduction in water activity.
    Appl Environ Microbiol. 1982 Jun;43(6):1501-3 PMID: 7103493
  35. Living with water stress: evolution of osmolyte systems.
    Science. 1982 Sep 24;217(4566):1214-22 PMID: 7112124
  36. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  37. Accumulation of trehalose and sucrose in cyanobacteria exposed to matric water stress.
    Appl Environ Microbiol. 1991 Mar;57(3):645-8 PMID: 16348431
  38. Glycine betaine transport in Escherichia coli: osmotic modulation.
    J Bacteriol. 1985 Jan;161(1):393-401 PMID: 3881395
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.15.5027-5035.1992
Published
1992-08-00
Pages
5027-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206317
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.15.5027-5035.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2