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PMID: 8487757 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression analysis of the mixed function oxidase system in rat brain by the polymerase chain reaction.

Molecular and cellular biochemistry ·Vol. 120 ·No. 2 ·1993-03-24 ·Pages 171-9

Hodgson AV, White TB, White JW, Strobel HW

Abstract

Metabolism of therapeutic drugs in the body by the mixed function oxidase system is an important consideration in the analysis of a drug's effectiveness. P450-dependent metabolism within the brain of a neuro-specific drug may affect the drug's course of action. To determine whether cytochrome P450 was expressed in brain, RNA was isolated from the whole brains of rats treated with a variety of known hepatic P450 inducers, including amitriptyline, imipramine, isosafrole, phenobarbital, and beta-naphthoflavone. The RNA was analyzed for the presence of P450 isozymes by the PCR technique. Differential expression of P450IA1, P450IIB1, P450IIB2, P450IID, and P450IIE1 was detected in the brain samples, depending on the treatment. Cytochrome P450 reductase expression was also detected in the brain samples, giving strong evidence that the brain contains a competent mixed function oxidase system under all conditions studied.

MeSH Terms
Amitriptyline/pharmacology Animals Base Sequence Benzoflavones/pharmacology Brain/drug effects,enzymology Cytochrome P-450 Enzyme System/biosynthesis,genetics Enzyme Induction/drug effects Female Imipramine/pharmacology Isoenzymes/biosynthesis,genetics Male Mixed Function Oxygenases/biosynthesis,genetics Molecular Sequence Data Nerve Tissue Proteins/biosynthesis,genetics Phenobarbital/pharmacology Polymerase Chain Reaction RNA, Messenger/biosynthesis Rats Rats, Sprague-Dawley/metabolism Safrole/pharmacology beta-Naphthoflavone
Chemicals
Benzoflavones Isoenzymes Nerve Tissue Proteins RNA, Messenger Amitriptyline beta-Naphthoflavone Cytochrome P-450 Enzyme System Mixed Function Oxygenases Imipramine Safrole isosafrole Phenobarbital
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hodgson A V
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225.
White T B
White J W
Strobel H W
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00926090
Published
1993-03-24
Pages
171-9
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
Grants
NIMH NIH HHS · MH44923 · United States
External Links
PubMed source DOI: 10.1007/BF00926090 View journal details
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