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PMID: 8444803 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular analysis of the 3,6-dideoxyhexose pathway genes of Yersinia pseudotuberculosis serogroup IIA.

Journal of bacteriology ·Vol. 175 ·No. 5 ·1993-03-00 ·Pages 1412-22

Kessler AC, Haase A, Reeves PR

Abstract

Salmonella enterica and Yersinia pseudotuberculosis are the only examples in nature known to use a variety of 3,6-dideoxyhexose derivatives as O antigen constituents. To allow a comparison of the responsible biosynthetic genes of the two organisms, we have sequenced a section of the Y. pseudotuberculosis serogroup IIA rfb region that contained the genes for the abequose biosynthetic pathway. Comparison of the identified genes with the rfb region of S. enterica LT2 showed that the two dideoxyhexose pathway gene clusters are related. The arrangement of the genes was largely conserved, and the G + C compositions of the two DNA regions were strikingly similar; however, the degree of conservation of nucleotide and protein sequences suggested that the two gene clusters have been evolving independently for considerable time. Hybridization experiments showed that the dideoxyhexose pathway genes are widespread throughout the various serogroups of Y. pseudotuberculosis.

MeSH Terms
Amino Acid Sequence Base Composition Base Sequence Codon DNA, Bacterial Genes, Bacterial Hexoses/genetics,metabolism Molecular Sequence Data Multigene Family Open Reading Frames Promoter Regions, Genetic Restriction Mapping Yersinia pseudotuberculosis/genetics,metabolism
Chemicals
Codon DNA, Bacterial Hexoses tyvelose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kessler A C
Department of Microbiology, University of Sydney, New South Wales, Australia.
Haase A
Reeves P R
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44 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.5.1412-1422.1993
Published
1993-03-00
Pages
1412-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC193228
Subset
IM
Databases
GENBANK
L01777
External Links
PubMed source DOI: 10.1128/jb.175.5.1412-1422.1993 PMC Full Text View journal details
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