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PMID: 8444464 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13.

Human genetics ·Vol. 90 ·No. 6 ·1993-02-00 ·Pages 577-83

Baldini A, Ried T, Shridhar V, Ogura K, D'Aiuto L, Rocchi M, Ward DC

Abstract

Using vector-CENP-B box polymerase chain reaction (PCR) we isolated and cloned from a human chromosome 21-specific plasmid library, a 1 kb DNA sequence, named p alpha H21. In in situ hybridization experiments, p alpha H21 hybridized, under high stringency conditions, to the centromeric region of all the human, chimpanzee, gorilla and orangutan chromosomes. On human chromosomes p alpha H21 also identified non-centromeric sequences at 2q21 (locus D2F33S1) and 9q13 (locus D9F33S2). The possible derivation of these sequences from ancestral centromeres is discussed. Sequence analysis confirmed the alphoid nature of the whole p alpha H21 insert.

MeSH Terms
Animals Base Sequence Blotting, Southern Centromere Chromosomes, Human, Pair 2 Chromosomes, Human, Pair 9 Conserved Sequence DNA, Satellite/analysis Female Gene Library Gorilla gorilla/genetics Hominidae/genetics Humans In Situ Hybridization, Fluorescence Male Molecular Sequence Data Pan troglodytes/genetics Polymerase Chain Reaction Pongo pygmaeus/genetics Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA
Chemicals
DNA, Satellite
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Baldini A
Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510.
Ried T
Shridhar V
Ogura K
D'Aiuto L
Rocchi M
Ward D C
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00202474
Published
1993-02-00
Pages
577-83
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
Grants
NHGRI NIH HHS · HG00030 · United States
NHGRI NIH HHS · HG00246 · United States
Databases
GENBANK
L13238, L13296, L13297, L14001, L14002, L14003, M64321, S70572, S70573, X66424
External Links
PubMed source DOI: 10.1007/BF00202474 View journal details
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