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PMID: 2040204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A chimpanzee-derived chromosome-specific alpha satellite DNA sequence conserved between chimpanzee and human.

Chromosoma ·Vol. 100 ·No. 3 ·1991-03-00 ·Pages 156-61

Baldini A, Miller DA, Miller OJ, Ryder OA, Mitchell AR

Abstract

We describe a cloned 2.7 kb alpha satellite sequence, Pan-3, from the pygmy chimpanzee (Pan paniscus) that specifically hybridizes in situ to chromosome 19 in the pygmy chimpanzee and to the homeologous human chromosome, no. 17. Using high stringency conditions of hybridization on Southern blots, this sequence hybridized to DNA from both species of chimpanzee (P. paniscus and P. troglodytes) and from human but not to DNA from gorilla (Gorilla gorilla) or orangutan (Pongo pygmaeus). Partial sequence analysis showed that Pan-3 and a previously described human chromosome 17-specific clone have up to 91% sequence identity. To our knowledge this is the highest sequence similarity reported between alphoid subsets from human and any other primate.

MeSH Terms
Animals Base Sequence Blotting, Southern Chromosomes, Human, Pair 17 DNA, Satellite/genetics Humans Male Molecular Sequence Data Pan troglodytes/genetics Sequence Homology, Nucleic Acid
Chemicals
DNA, Satellite
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Baldini A
Department of Molecular Biology and Genetics, Wayne State University, Detroit, MI 48201.
Miller D A
Miller O J
Ryder O A
Mitchell A R
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00337244
Published
1991-03-00
Pages
156-61
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM 34959 · United States
External Links
PubMed source DOI: 10.1007/BF00337244 View journal details
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