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PMID: 3475246 Published · ppublish English Journal Article

Application of cloned satellite DNA sequences to molecular-cytogenetic analysis of constitutive heterochromatin heteromorphisms in man.

Human genetics ·Vol. 76 ·No. 2 ·1987-06-00 ·Pages 157-64

Yurov YB, Mitkevich SP, Alexandrov IA

Abstract

The cloned alpha-satellite DNA sequences were used to evaluate the specificity and possible variability of repetitive DNA in constitutive heterochromatin of human chromosomes. Five probes with high specificity to individual chromosomes (chromosomes 3, 11, 17, 18, and X) were in situ hybridized to metaphase chromosomes of different individuals. The stable position of alpha-satellite DNA sequences in heterochromatic regions of particular chromosomes was found. Therefore, the chromosome-specific alpha-satellite DNA sequences may be used as molecular markers for heterochromatic regions of certain human chromosomes. The homologous chromosomes of many individuals were characterized by cytologically visible heteromorphisms of hybridization intensity with chromosome-specific alpha-satellite DNA sequences. A special analysis of hybridization between homologues with morphological differences provided the evidence for a high resolution power of the in situ hybridization technique for evaluation of chromosome heteromorphisms. The approaches for detection of heteromorphisms in cases without morphological differences between homologues are discussed. The results obtained indicate that constitutive heterochromatin of human chromosomes has a variable amount of alpha-satellite DNA sequences. In situ hybridization of cloned satellite DNA sequences may be used as a new general approach to analysis of chromosome heteromorphisms in man.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Human, Pair 11 Chromosomes, Human, Pair 17 Chromosomes, Human, Pair 18 Chromosomes, Human, Pair 3 Cloning, Molecular DNA, Satellite/genetics Genetic Markers Heterochromatin/genetics Humans Karyotyping Nucleic Acid Hybridization Polymorphism, Genetic X Chromosome
Chemicals
DNA, Satellite Genetic Markers Heterochromatin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yurov Y B
Mitkevich S P
Alexandrov I A
References (15)
15 references, click to expand
  1. [Chromosome organization of human alphoid DNA].
    Dokl Akad Nauk SSSR. 1986 May-Jun;288(1):242-5 PMID: 3458583
  2. Aspects of evaluation, significance, and evolution of human C-band heteromorphism.
    Hum Genet. 1982;61(4):281-94 PMID: 6759365
  3. Cloning of human satellite III DNA: different components are on different chromosomes.
    Nucleic Acids Res. 1979 Jul 25;6(10):3177-97 PMID: 573470
  4. A rapid screening test for antenatal sex determination.
    Lancet. 1984 Jan 7;1(8367):14-6 PMID: 6140342
  5. 18p- syndrome: an unusual case and diagnosis by in situ hybridization with chromosome 18-specific alphoid DNA sequence.
    Hum Genet. 1986 Feb;72(2):185-7 PMID: 3753696
  6. [Cloned fragment of human alphoid DNA--a molecular marker of the pericentromeric region of chromosome 18].
    Genetika. 1986 May;22(5):868-76 PMID: 3460927
  7. Highly repeated sequences in mammalian genomes.
    Int Rev Cytol. 1982;76:67-112 PMID: 6749748
  8. A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomes.
    Cytogenet Cell Genet. 1978;22(1-6):352-6 PMID: 752501
  9. Characterization of a cloned repetitive DNA sequence concentrated on the human X chromosome.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6593-7 PMID: 6959140
  10. Localisation of a male-specific DNA fragment to a sub-region of the human Y chromosome.
    Nature. 1978 Mar 23;272(5651):324-8 PMID: 76288
  11. Chromosome-specific organization of human alpha satellite DNA.
    Am J Hum Genet. 1985 May;37(3):524-32 PMID: 2988334
  12. Chromosomal localization of complex and simple repeated human DNAs.
    Chromosoma. 1978 Mar 22;66(1):23-32 PMID: 639625
  13. [Optimization of the conditions for the in situ hybridization of cloned DNA sequences and for the differential staining of human chromosomes].
    Biull Eksp Biol Med. 1984 May;97(5):595-8 PMID: 6722340
  14. Human chromosome heteromorphisms (variants).
    Prog Med Genet. 1977;2:251-74 PMID: 897201
  15. A cloned repeated DNA sequence in human chromosome heteromorphisms.
    Cytogenet Cell Genet. 1981;29(1):32-9 PMID: 6161756
Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00284914
Published
1987-06-00
Pages
157-64
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00284914 View journal details
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