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PMID: 3658703 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence heterogeneity of alpha satellite repetitive DNA: a survey of alphoid sequences from different human chromosomes.

Nucleic acids research ·Vol. 15 ·No. 18 ·1987-09-25 ·Pages 7549-69

Waye JS, Willard HF

Abstract

The human alpha satellite DNA family is composed of diverse, tandemly reiterated monomer units of approximately 171 basepairs localized to the centromeric region of each chromosome. These sequences are organized in a highly chromosome-specific manner with many, if not all human chromosomes being characterized by individually distinct alphoid subsets. Here, we compare the nucleotide sequences of 153 monomer units, representing alphoid components of at least 12 different human chromosomes. Based on the analysis of sequence variation at each position within the 171 basepair monomer, we have derived a consensus sequence for the monomer unit of human alpha satellite DNA which we suggest may reflect the monomer sequence from which different chromosomal subsets have evolved. Sequence heterogeneity is evident at each position within the consensus monomer unit and there are no positions of strict nucleotide sequence conservation, although some regions are more variable than others. A substantial proportion of the overall sequence variation may be accounted for by nucleotide changes which are characteristic of monomer components of individual chromosomal subsets or groups of subsets which have a common evolutionary history.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Human DNA, Satellite/genetics Heterochromatin/analysis Humans Molecular Sequence Data Phylogeny Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid
Chemicals
DNA, Satellite Heterochromatin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Waye J S
Department of Medical Genetics, University of Toronto, Ontario, Canada.
Willard H F
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47 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.18.7549
Published
1987-09-25
Pages
7549-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306267
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.18.7549 PMC Full Text View journal details
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