Home LiteratureArticle Details
PMID: 8441675 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The adenovirus DNA binding protein effects the kinetics of DNA replication by a mechanism distinct from NFI or Oct-1.

Nucleic acids research ·Vol. 21 ·No. 3 ·1993-02-11 ·Pages 641-7

Mul YM, van der Vliet PC

Abstract

Initiation of adenovirus DNA replication in vitro minimally requires the viral TP-DNA template and the precursor terminal protein-DNA polymerase heterodimer (pTP-pol). Optimal initiation occurs in the presence of the cellular transcription factors NFI and Oct-1 and the viral DNA binding protein (DBP). We have studied the influence of these three stimulatory proteins on the kinetics of formation of the pTP-dCMP initiation complex. NFI increases the Vmax of the reaction but does not affect the apparent Km for dC-TP. This indicates that NFI acts by enlarging the amount of active initiation complex in agreement with its stabilizing effect on binding of pTP-pol to the template. Similar kinetic effects were observed for Oct-1. Since Oct-1 does not stabilize binding of pTP-pol to the origin this suggests that Oct-1 increases the rate of pTP-dCMP formation. DBP stimulates the initiation reaction in two ways. First, it moderately increases the Vmax at suboptimal NFI concentrations, which is related to its enhancing effect on binding of NFI to the origin. Second, a much larger stimulation was caused by DBP itself based on a reduction of the Km for dCTP, which was independent of the concentration of pTP-pol or NFI. The Km for dCTP during initiation is lower than during elongation.

MeSH Terms
Adenoviridae/genetics,metabolism Amino Acid Sequence CCAAT-Enhancer-Binding Proteins Cytidine Triphosphate/metabolism DNA Replication DNA, Viral/biosynthesis,metabolism DNA-Binding Proteins/metabolism Host Cell Factor C1 Kinetics Molecular Sequence Data NFI Transcription Factors Octamer Transcription Factor-1 Transcription Factors/metabolism
Chemicals
CCAAT-Enhancer-Binding Proteins DNA, Viral DNA-Binding Proteins Host Cell Factor C1 NFI Transcription Factors Octamer Transcription Factor-1 Transcription Factors Cytidine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mul Y M
Laboratory for Physiological Chemistry, University of Utrecht, The Netherlands.
van der Vliet P C
References (63)
63 references, click to expand
  1. Transition from initiation to elongation in protein-primed phi 29 DNA replication: salt-dependent stimulation by the viral protein p6.
    J Virol. 1988 Nov;62(11):4167-72 PMID: 3172342
  2. POU domain transcription factors from different subclasses stimulate adenovirus DNA replication.
    Nucleic Acids Res. 1992 Dec 11;20(23):6369-75 PMID: 1475198
  3. Purification of a NF1-like DNA-binding protein from rat liver and cloning of the corresponding cDNA.
    EMBO J. 1988 Oct;7(10):3115-23 PMID: 3053160
  4. Complex formation between the adenovirus type 5 DNA-binding protein and single-stranded DNA.
    Eur J Biochem. 1978 May 16;86(2):389-98 PMID: 26566
  5. Interaction between the adenovirus DNA-binding protein and double-stranded DNA.
    J Mol Biol. 1979 Aug 5;132(2):163-80 PMID: 231660
  6. Adenovirus coded deoxyribonucleic acid binding protein. Isolation, physical properties, and effects of proteolytic digestion.
    Biochemistry. 1980 Jun 10;19(12):2802-10 PMID: 6249341
  7. Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6438-42 PMID: 6216480
  8. Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6177-81 PMID: 6336326
  9. Structure and function of the adenovirus origin of replication.
    Cell. 1984 May;37(1):309-19 PMID: 6722875
  10. Protein-primed replication of plasmids containing the terminus of the adenovirus genome. II. Purification and characterization of a host protein required for the replication of DNA templates devoid of the terminal protein.
    J Biol Chem. 1984 Jun 25;259(12):7815-25 PMID: 6330083
  11. Properties of the adenovirus DNA polymerase.
    J Biol Chem. 1984 Aug 10;259(15):9487-95 PMID: 6540263
  12. The origin of adenovirus DNA replication: minimal DNA sequence requirement in vivo.
    EMBO J. 1985 Feb;4(2):421-6 PMID: 4018031
  13. Adenovirus DNA replication in vitro: site-directed mutagenesis of the nuclear factor I binding site of the Ad2 origin.
    Nucleic Acids Res. 1985 Jul 11;13(13):4935-52 PMID: 4040630
  14. Recognition site of nuclear factor I, a sequence-specific DNA-binding protein from HeLa cells that stimulates adenovirus DNA replication.
    EMBO J. 1985 Jun;4(6):1515-21 PMID: 4040852
  15. Characterization of the chymotryptic core of the adenovirus DNA-binding protein.
    FEBS Lett. 1985 Sep 2;188(2):248-52 PMID: 4040872
  16. Origin of adenovirus DNA replication. Role of the nuclear factor I binding site in vivo.
    J Mol Biol. 1985 Nov 5;186(1):129-36 PMID: 4078897
  17. Reconstruction of adenovirus replication origins with a human nuclear factor I binding site.
    J Biol Chem. 1986 Mar 5;261(7):3339-46 PMID: 3005289
  18. A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts.
    Nucleic Acids Res. 1986 Feb 11;14(3):1303-17 PMID: 3513122
  19. Replication of phage phi 29 DNA in vitro: role of the viral protein p6 in initiation and elongation.
    Nucleic Acids Res. 1986 Jun 25;14(12):4923-37 PMID: 3088545
  20. The adenovirus DNA binding protein and adenovirus DNA polymerase interact to catalyze elongation of primed DNA templates.
    J Biol Chem. 1986 Aug 5;261(22):10218-27 PMID: 2942538
  21. Single-stranded DNA binding proteins required for DNA replication.
    Annu Rev Biochem. 1986;55:103-36 PMID: 3527040
  22. DNA precursor pools and ribonucleotide reductase activity: distribution between the nucleus and cytoplasm of mammalian cells.
    Mol Cell Biol. 1985 Dec;5(12):3443-50 PMID: 3915777
  23. Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication.
    Nature. 1986 Aug 14-20;322(6080):656-9 PMID: 3748145
  24. Analysis of nuclear factor I binding to DNA using degenerate oligonucleotides.
    Nucleic Acids Res. 1986 Nov 25;14(22):9117-32 PMID: 3786147
  25. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  26. Adenovirus origin of DNA replication: sequence requirements for replication in vitro.
    Mol Cell Biol. 1987 Feb;7(2):864-74 PMID: 3821730
  27. Sequence-specific interactions between cellular DNA-binding proteins and the adenovirus origin of DNA replication.
    Mol Cell Biol. 1987 Feb;7(2):875-86 PMID: 3821731
  28. Contactpoint analysis of the HeLa nuclear factor I recognition site reveals symmetrical binding at one side of the DNA helix.
    EMBO J. 1987 Jan;6(1):161-8 PMID: 3034574
  29. Interaction between adenovirus DNA-binding protein and single-stranded polynucleotides studied by circular dichroism and ultraviolet absorption.
    Biochemistry. 1987 Jul 28;26(15):4646-52 PMID: 3663614
  30. Interaction of the bacteriophage phi 29 protein p6 with double-stranded DNA.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):314-8 PMID: 3124105
  31. A quantitative analysis of nuclear factor I/DNA interactions.
    Nucleic Acids Res. 1988 May 25;16(10):4419-35 PMID: 3380685
  32. Initiation of adenovirus DNA replication. II. Structural requirements using synthetic oligonucleotide adenovirus templates.
    J Biol Chem. 1988 Jul 15;263(20):9809-17 PMID: 2838480
  33. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.
    Nature. 1988 Jul 21;334(6179):218-24 PMID: 3398920
  34. Multiple genes encode nuclear factor 1-like proteins that bind to the promoter for 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8963-7 PMID: 3194401
  35. Recognition mechanisms in the synthesis of animal virus DNA.
    Biochem J. 1989 Feb 15;258(1):3-16 PMID: 2649089
  36. Intracellular organization of DNA precursor biosynthetic enzymes.
    Trends Biochem Sci. 1988 Oct;13(10):394-7 PMID: 3072702
  37. The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.
    Cell. 1989 Aug 25;58(4):741-53 PMID: 2504497
  38. Co-operative interactions between NFI and the adenovirus DNA binding protein at the adenovirus origin of replication.
    EMBO J. 1989 Jun;8(6):1841-8 PMID: 2767055
  39. Signals at the bacteriophage phi 29 DNA replication origins required for protein p6 binding and activity.
    EMBO J. 1989 Jun;8(6):1879-85 PMID: 2767056
  40. Animal virus DNA replication.
    Annu Rev Biochem. 1989;58:671-717 PMID: 2549858
  41. Mechanism of inhibition of adenovirus DNA replication by the acyclic nucleoside triphosphate analogue (S)-HPMPApp: influence of the adenovirus DNA binding protein.
    Nucleic Acids Res. 1989 Nov 25;17(22):8917-29 PMID: 2587248
  42. Adenovirus DNA-binding protein forms a multimeric protein complex with double-stranded DNA and enhances binding of nuclear factor I.
    J Virol. 1990 Jan;64(1):379-86 PMID: 2293667
  43. Initiation of eukaryotic DNA replication in vitro.
    Annu Rev Cell Biol. 1989;5:197-245 PMID: 2557059
  44. Structural and functional organization of a porcine gene coding for nuclear factor I.
    Biochemistry. 1989 Oct 3;28(20):8191-200 PMID: 2513876
  45. Adenovirus subviral particles and cores can support limited DNA replication.
    J Gen Virol. 1989 Dec;70 ( Pt 12):3235-48 PMID: 2607337
  46. Amino-terminal domain of NF1 binds to DNA as a dimer and activates adenovirus DNA replication.
    EMBO J. 1990 Feb;9(2):559-66 PMID: 2303041
  47. Chicken NFI/TGGCA proteins are encoded by at least three independent genes: NFI-A, NFI-B and NFI-C with homologues in mammalian genomes.
    Nucleic Acids Res. 1990 May 11;18(9):2607-16 PMID: 2339052
  48. A novel nucleoprotein complex at a replication origin.
    Science. 1990 May 25;248(4958):1012-6 PMID: 2111580
  49. The DNA binding domain (POU domain) of transcription factor oct-1 suffices for stimulation of DNA replication.
    EMBO J. 1990 Jun;9(6):1883-8 PMID: 2347308
  50. The highly conserved amino acid sequence motif Tyr-Gly-Asp-Thr-Asp-Ser in alpha-like DNA polymerases is required by phage phi 29 DNA polymerase for protein-primed initiation and polymerization.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4610-4 PMID: 2191296
  51. Protein-protein interactions between adenovirus DNA polymerase and nuclear factor I mediate formation of the DNA replication preinitiation complex.
    J Biol Chem. 1990 Oct 25;265(30):18634-42 PMID: 2211726
  52. Transcription factors NFI and NFIII/oct-1 function independently, employing different mechanisms to enhance adenovirus DNA replication.
    J Virol. 1990 Nov;64(11):5510-8 PMID: 2214023
  53. Regulation of transcription and cell identity by POU domain proteins.
    Cell. 1991 Feb 8;64(3):475-8 PMID: 1991317
  54. Interactions between the adenovirus type 2 DNA polymerase and the DNA binding domain of nuclear factor I.
    New Biol. 1990 Dec;2(12):1083-90 PMID: 2088500
  55. Mutagenesis of conserved region I in the DNA polymerase from human adenovirus serotype 2.
    Virology. 1991 Sep;184(1):235-41 PMID: 1871969
  56. Protein-priming of DNA replication.
    Annu Rev Biochem. 1991;60:39-71 PMID: 1883199
  57. Enhancement of DNA replication by transcription factors NFI and NFIII/Oct-1 depends critically on the positions of their binding sites in the adenovirus origin of replication.
    Biochim Biophys Acta. 1991 Aug 27;1090(1):61-9 PMID: 1883843
  58. POU proteins bend DNA via the POU-specific domain.
    EMBO J. 1991 Oct;10(10):3007-14 PMID: 1915275
  59. Identification of a fourth nuclear factor I gene in chicken by cDNA cloning: NFI-X.
    Nucleic Acids Res. 1991 Dec 11;19(23):6641 PMID: 1754401
  60. Nuclear factor I enhances adenovirus DNA replication by increasing the stability of a preinitiation complex.
    EMBO J. 1992 Feb;11(2):751-60 PMID: 1537346
  61. Recognition of the adenovirus type 2 origin of DNA replication by the virally encoded DNA polymerase and preterminal proteins.
    EMBO J. 1992 Feb;11(2):761-8 PMID: 1537347
  62. Structural alterations of double-stranded DNA in complex with the adenovirus DNA-binding protein. Implications for its function in DNA replication.
    J Mol Biol. 1992 Jun 20;225(4):999-1011 PMID: 1319498
  63. Interactions between deoxyribonucleotide and DNA synthesis.
    Annu Rev Biochem. 1988;57:349-74 PMID: 3052277
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.3.641
Published
1993-02-11
Pages
641-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309164
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/21.3.641 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2