Abstract
Using an expression cDNA cloning approach, we examined human tumor cell lines for novel oncogenes that might evade detection by conventional techniques. We isolated a transforming sequence that was highly efficient in transforming NIH 3T3 mouse fibroblasts. DNA sequence analysis identified the gene as the human homolog of a recently cloned alpha subunit of mouse GTP-binding protein G alpha 12. NIH 3T3 cells transfected with G alpha 12 cDNA grew in soft agar and were tumorigenic in nude mice. There were no apparent mutations in the cloned cDNA in comparison with a G alpha 12 cDNA clone isolated from a normal human epithelial cell library, implying that overexpression alone was sufficient to cause NIH 3T3 cell transformation. The observed altered growth properties mediated by G alpha 12 showed a certain degree of dependency on serum factors, and its mitogenic potential was also potently inhibited by suramin treatment.
MeSH Terms
3T3 Cells
Amino Acid Sequence
Animals
Blotting, Northern
Cell Transformation, Neoplastic/genetics
Cloning, Molecular
DNA/biosynthesis,drug effects
Electrophoresis, Polyacrylamide Gel
GTP-Binding Proteins/genetics,physiology
Gene Library
Humans
Mice
Molecular Sequence Data
Oncogenes
Sequence Homology, Amino Acid
Suramin/pharmacology
Tumor Cells, Cultured
Chemicals
Suramin
DNA
GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chan A M
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
Fleming T P
McGovern E S
Chedid M
Miki T
Aaronson S A
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