Abstract
In a recently discovered class of oncogenes, GTPase-inhibiting mutations constitutively activate alpha subunits of signal-transducing guanine nucleotide-binding proteins (G proteins). Somatic mutations in a subclass of endocrine tumors are found in the arginine-179 codon of the alpha subunit of Gi2 (alpha i2), creating the putative gip2 oncogene. We have tested the ability of gip2 to mediate neoplastic transformation of Rat-1 and NIH 3T3 fibroblasts in tissue culture. Expression of a mutant alpha i2 cDNA encoding cysteine in place of arginine-179 (alpha i2-R179C) caused Rat-1 cells to grow to a higher density in monolayer culture, to lose anchorage dependence, and to form tumors when injected subcutaneously into nude mice. In contrast, expression of alpha i2-R179C failed to alter growth or tumorigenicity of NIH 3T3 cells. We conclude that gip2 is an oncogene, by the criterion that it induces neoplastic transformation of Rat-1 cells. Failure of gip2 to transform NIH 3T3 cells is in keeping with clinical indications that gip2 is a tissue-selective oncogene.
MeSH Terms
Animals
Cell Division/drug effects
Cell Line
Cell Transformation, Neoplastic
Cyclic AMP/pharmacology
GTP-Binding Proteins/biosynthesis,genetics,isolation & purification
Genes, ras
Macromolecular Substances
Mice
Mice, Nude
Mutation
Neoplasms, Experimental/genetics,pathology
Oncogenes
Rats
Recombinant Proteins/biosynthesis,isolation & purification
Transplantation, Heterologous
Chemicals
Macromolecular Substances
Recombinant Proteins
Cyclic AMP
GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pace A M
Department of Pharmacology, University of California, San Francisco 94143.
Wong Y H
Bourne H R
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