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PMID: 8413182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Osmoregulation of the fatty acid receptor gene fadL in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 240 ·No. 3 ·1993-09-00 ·Pages 339-47

Higashitani A, Nishimura Y, Hara H, Aiba H, Mizuno T, Horiuchi K

Abstract

The fadL gene of Escherichia coli codes for an outer membrane protein that is involved in the uptake of long-chain fatty acids. Uptake is regulated by environmental osmolarity, and decreases when the cells are grown under conditions of high osmolarity. A temperature-sensitive mutant that requires fatty acid for growth at 42 degrees C was unable to grow at the high temperature even in the presence of fatty acid if the medium contained 10% sucrose. Promoter activity of the fadL gene in vivo was repressed by high osmolarity in a FadR repressor null mutant. Furthermore, in vitro transcription of the fadL gene was strongly repressed by the addition of OmpR and EnvZ proteins. The results of gel retardation and DNase I protection experiments indicated that OmpR, after incubation with the protein kinase EnvZ, specifically binds to at least four sites around the fadL promoter, two upstream and two downstream from the transcriptional start site. These results suggest that transcription of the fadL gene is osmotically regulated by the OmpR-EnvZ two-component system.

Related Genes
fabB fadL
MeSH Terms
Bacterial Outer Membrane Proteins/genetics Base Sequence Binding Sites DNA, Bacterial Escherichia coli/genetics,growth & development,metabolism Escherichia coli Proteins Fatty Acid Transport Proteins Fatty Acids/metabolism Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Oleic Acid Oleic Acids/metabolism Osmolar Concentration Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins DNA, Bacterial Escherichia coli Proteins Fatty Acid Transport Proteins Fatty Acids Oleic Acids fadL protein, E coli Oleic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Higashitani A
Department of Microbial Genetics, National Institute of Genetics, Shizuoka-ken, Japan.
Nishimura Y
Hara H
Aiba H
Mizuno T
Horiuchi K
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00280384
Published
1993-09-00
Pages
339-47
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00280384
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