Home LiteratureArticle Details
PMID: 3286621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The fadL gene product of Escherichia coli is an outer membrane protein required for uptake of long-chain fatty acids and involved in sensitivity to bacteriophage T2.

Journal of bacteriology ·Vol. 170 ·No. 6 ·1988-06-00 ·Pages 2850-4

Black PN

Abstract

The fadL+ gene of Escherichia coli encodes an outer membrane protein (FadL) essential for the uptake of long-chain fatty acids (C12 to C18). The present study shows that in addition to being required for uptake of and growth on the long-chain fatty acid oleate (C18:1), FadL acts as a receptor of bacteriophage T2. Bacteriophage T2-resistant (T2r) strains lacked FadL and were unable to take up and grow on long-chain fatty acids. Upon transformation with the fadL+ clone pN103, T2r strains became sensitive to bacteriophage T2 (T2s), became able to take up long-chain fatty acids at wild-type levels, and contained FadL in the outer membrane.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics,metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Fatty Acid Transport Proteins Fatty Acids/metabolism Immunosorbent Techniques Oleic Acid Oleic Acids/metabolism T-Phages/pathogenicity
Chemicals
Bacterial Outer Membrane Proteins Escherichia coli Proteins Fatty Acid Transport Proteins Fatty Acids Oleic Acids fadL protein, E coli Oleic Acid
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Black P N
Department of Biochemistry, College of Medicine, University of Tennessee, Memphis 38163.
References (34)
34 references, click to expand
  1. Molecular basis of bacterial outer membrane permeability.
    Microbiol Rev. 1985 Mar;49(1):1-32 PMID: 2580220
  2. Long-chain fatty acid transport in Escherichia coli. Cloning, mapping, and expression of the fadL gene.
    J Biol Chem. 1985 Feb 10;260(3):1780-9 PMID: 3881440
  3. Pathways for the incorporation of exogenous fatty acids into phosphatidylethanolamine in Escherichia coli.
    J Biol Chem. 1985 Oct 15;260(23):12720-4 PMID: 3900077
  4. Transport of long-chain fatty acids in Escherichia coli. Evidence for role of fadL gene product as long-chain fatty acid receptor.
    J Biol Chem. 1986 Jan 5;261(1):167-71 PMID: 3001045
  5. Peptidoglycan association of bacteriophage T5 receptor in Escherichia coli K-12.
    J Bacteriol. 1986 Jun;166(3):1137-40 PMID: 3519578
  6. Transfer of fatty acids from the 1-position of phosphatidylethanolamine to the major outer membrane lipoprotein of Escherichia coli.
    J Biol Chem. 1986 Aug 25;261(24):11328-33 PMID: 3525566
  7. New locus (ttr) in Escherichia coli K-12 affecting sensitivity to bacteriophage T2 and growth on oleate as the sole carbon source.
    J Bacteriol. 1986 Nov;168(2):534-40 PMID: 3536849
  8. Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli.
    J Biol Chem. 1987 Jan 25;262(3):1412-9 PMID: 3027089
  9. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  10. The induction of the enzymes of fatty acid degradation in Escherichia coli.
    Biochem Biophys Res Commun. 1967 Oct 11;29(1):28-33 PMID: 4861587
  11. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  12. Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants.
    Eur J Biochem. 1971 Apr;19(3):442-50 PMID: 4928881
  13. Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding.
    J Biol Chem. 1974 Dec 25;249(24):8019-29 PMID: 4609976
  14. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  15. In vitro competition between ferrichrome and phage for the outer membrane T5 receptor complex of Escherichia coli.
    Biochem Biophys Res Commun. 1975 May 19;64(2):687-93 PMID: 1096882
  16. Phage T6--colicin K receptor and nucleoside transport in Escherichia coli.
    FEBS Lett. 1976 Nov;70(1):109-12 PMID: 791677
  17. Transport of long-chain fatty acids by Escherichia coli: mapping and characterization of mutants in the fadL gene.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3377-81 PMID: 356053
  18. Major outer membrane proteins of E. coli K12 serve as receptors for the phages T2 (protein Ia) and 434 (protein Ib).
    Mol Gen Genet. 1978 Aug 17;164(2):131-5 PMID: 360042
  19. Receptor for bacteriophage lambda of Escherichia coli forms larger pores in black lipid membranes than the matrix protein (porin).
    J Bacteriol. 1979 Apr;138(1):33-9 PMID: 374375
  20. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  21. Kinetics of the utilization of medium and long chain fatty acids by mutant of Escherichia coli defective in the fadL gene.
    J Biol Chem. 1979 Sep 25;254(18):9130-4 PMID: 383713
  22. Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):167-71 PMID: 6444720
  23. Regulation of fatty acid degradation in Escherichia coli: isolation and characterization of strains bearing insertion and temperature-sensitive mutations in gene fadR.
    J Bacteriol. 1980 May;142(2):621-32 PMID: 6247326
  24. Precursor proteins are intermediates in vivo in the synthesis of two major outer membrane proteins, the OmpA and OmpF proteins, of Escherichia coli K12.
    Eur J Biochem. 1981 Jan;113(2):375-80 PMID: 7009158
  25. Transport of long and medium chain fatty acids by Escherichia coli K12.
    J Biol Chem. 1981 Apr 25;256(8):3735-42 PMID: 7012142
  26. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  27. Sequence information within the lamB genes in required for proper routing of the bacteriophage lambda receptor protein to the outer membrane of Escherichia coli K-12.
    J Mol Biol. 1982 Mar 25;156(1):93-112 PMID: 6212690
  28. Microbial envelope proteins related to iron.
    Annu Rev Microbiol. 1982;36:285-309 PMID: 6293371
  29. Cytodifferentiation in the accessory glands of Tenebrio molitor. VIII. Crossed immunoelectrophoretic analysis of terminal differentiation in the postecdysial tubular accessory glands.
    Dev Biol. 1982 Nov;94(1):106-15 PMID: 7152098
  30. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  31. Regulation of fatty acid transport in Escherichia coli: analysis by operon fusion.
    J Bacteriol. 1983 Sep;155(3):1450-4 PMID: 6350271
  32. Two-step resistance by Escherichia coli B to bacteriophage T2.
    Genetics. 1984 May;107(1):1-7 PMID: 6373494
  33. Transport of long chain fatty acids in Escherichia coli. Identification of a membrane protein associated with the fadL gene.
    J Biol Chem. 1984 Jul 10;259(13):8437-43 PMID: 6376508
  34. Demonstration of a bacteriophage receptor site on the Escherichia coli K12 outer-membrane protein OmpC by the use of a protease.
    Eur J Biochem. 1985 Jul 1;150(1):161-9 PMID: 3894021
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.6.2850-2854.1988
Published
1988-06-00
Pages
2850-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211212
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.6.2850-2854.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2