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PMID: 8407839 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens.

Journal of bacteriology ·Vol. 175 ·No. 20 ·1993-10-00 ·Pages 6614-25

Charles TC, Nester EW

Abstract

TnphoA mutagenesis of Agrobacterium tumefaciens identified new extracytoplasmic protein-encoding virulence loci. Mutations in these loci conferred increased sensitivity to detergents and several antibiotics. Clones carrying these loci were isolated from an A. tumefaciens cosmid library by complementation of the detergent sensitivities of the mutants. The locus on one complementing clone was delineated by Tn5 and TnphoA mutagenesis. DNA sequence analysis of the delineated region revealed that this locus is made up of two transcriptional units, chvG and chvI, which were predicted, on the basis of amino acid sequence homology, to encode the members of a two-component sensory transduction system. The membrane-spanning sensor, a histidine protein kinase, was designated ChvG, and the response regulator, presumably a transcriptional activator, was designated ChvI. Surprisingly, ChvG was also predicted to contain a Walker type A consensus nucleotide binding site, which is unusual for sensor histidine protein kinases. Site-specific insertion mutations in either chvG or chvI abolished tumor formation ability, as well as the ability to grow on complex media. Neither the genes which are regulated nor the inducing signal is known yet for this system.

Related Genes
chvG chvI pckA phoR virE
MeSH Terms
Agrobacterium tumefaciens/genetics,pathogenicity Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics Bacterial Proteins/chemistry,genetics Base Sequence Chromosomes, Bacterial/ultrastructure DNA Primers/chemistry DNA Transposable Elements Genes, Bacterial Genetic Complementation Test Genetic Linkage Histidine Kinase Membrane Proteins/genetics Molecular Sequence Data Mutagenesis Plant Diseases Protein Kinases/genetics Restriction Mapping Sequence Alignment Transcription Factors/genetics
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA Primers DNA Transposable Elements Membrane Proteins Transcription Factors chvI protein, Bacteria Protein Kinases Histidine Kinase chvG protein, Bacteria
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Charles T C
Department of Microbiology, University of Washington, Seattle 98195.
Nester E W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.20.6614-6625.1993
Published
1993-10-00
Pages
6614-25
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206773
Subset
IM
Grants
NIGMS NIH HHS · GM32618-18 · United States
Databases
GENBANK
L18860
External Links
PubMed source DOI: 10.1128/jb.175.20.6614-6625.1993 PMC Full Text View journal details
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