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PMID: 1909032 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mechanism of phenolic activation of Agrobacterium virulence genes: development of a specific inhibitor of bacterial sensor/response systems.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 17 ·1991-09-01 ·Pages 7854-8

Hess KM, Dudley MW, Lynn DG, Joerger RD, Binns AN

Abstract

The aglycone of the dihydrodiconiferyl alcohol glycosides, a series of phenolic growth factors able to substitute for some of the hormone requirements of tobacco cell division, are also potent inducers of virulence gene expression in Agrobacterium tumefaciens. However, these factors do not conform to the previously established structural requirements necessary for vir expression. Systematic evaluation of the structural requirements of these inducers has led to a model detailing the role of the phenolics in induction. With this model, a specific inhibitor of vir induction has been developed. This inhibitor does not affect the induction of other genes on the Ti plasmid but irreversibly blocks vir expression. The inhibitor has been used to show that the inducing phenolics must be constantly present to maintain expression of the vir regulon.

Related Genes
occ vir
MeSH Terms
Gene Expression Regulation, Viral/drug effects Genes, Bacterial Growth Substances/chemical synthesis,pharmacology Kinetics Molecular Structure Phenols/chemical synthesis,pharmacology Plasmids/drug effects Rhizobium/drug effects,genetics,pathogenicity Structure-Activity Relationship Virulence/genetics beta-Galactosidase/genetics,metabolism
Chemicals
Growth Substances Phenols beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hess K M
Searle Chemistry Laboratory, University of Chicago, IL 60637.
Dudley M W
Lynn D G
Joerger R D
Binns A N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.17.7854
Published
1991-09-01
Pages
7854-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52402
Subset
IM
Grants
NIGMS NIH HHS · GM33585 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.17.7854 PMC Full Text View journal details
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