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PMID: 8406871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of the 98-kilodalton iron-regulated outer membrane protein of Neisseria meningitidis.

Infection and immunity ·Vol. 61 ·No. 11 ·1993-11-00 ·Pages 4724-33

Pettersson A, van der Ley P, Poolman JT, Tommassen J

Abstract

When grown under iron limitation, Neisseria meningitidis expresses several additional outer membrane proteins (OMPs), which were studied to assess their vaccine potential. Two monoclonal antibodies were obtained against a 98-kDa OMP of strain 2996 (B:2b:P1.2). Cross-reactivity studies revealed that the two antibodies reacted with 44 and 42 of 74 meningococcal strains, respectively. The antibodies did not block the binding of transferrin or lactoferrin to intact cells. The structural gene for the protein, tentatively designated iroA, was isolated and sequenced. Computer analysis revealed homology to the ferric siderophore receptors in the outer membrane of Escherichia coli and to gonococcal transferrin-binding protein 1 (TbpA). The high degree of cross-reactivity and the results of Southern blot analyses, which showed that the iroA gene is also present in strains that did not react with the monoclonal antibodies, suggest that the 98-kDa OMP is well conserved among meningococci and that it is a suitable vaccine candidate. However, the antibodies were not bactericidal in an in vitro assay with human complement.

Related Genes
iroA
MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Bacterial Outer Membrane Proteins/chemistry,genetics,immunology Base Sequence Cloning, Molecular Genes, Bacterial Iron/pharmacology Lactoferrin/metabolism Mice Mice, Inbred BALB C Molecular Sequence Data Molecular Weight Neisseria meningitidis/chemistry,genetics Sequence Alignment Transferrin/metabolism
Chemicals
Antibodies, Monoclonal Bacterial Outer Membrane Proteins Transferrin iroA protein, bacteria Iron Lactoferrin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pettersson A
Department of Molecular Cell Biology, Utrecht University, The Netherlands.
van der Ley P
Poolman J T
Tommassen J
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48 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.11.4724-4733.1993
Published
1993-11-00
Pages
4724-33
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281227
Subset
IM
Databases
GENBANK
X69214
External Links
PubMed source DOI: 10.1128/iai.61.11.4724-4733.1993 PMC Full Text View journal details
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