Home LiteratureArticle Details
PMID: 6783544 Published · ppublish English Journal Article

Energy-independent uptake of iron from citrate by isolated outer membranes of Neisseria meningitidis.

Infection and immunity ·Vol. 31 ·No. 2 ·1981-02-00 ·Pages 547-53

Simonson C, Trivett T, DeVoe IW

Abstract

Cyanide-poisoned Neisseria meningitidis SD1C cells rapidly took up 55Fe from iron-citrate complexes during the first 2 min, after which no further iron was accumulated. [14C]citrate was not taken up concomitantly with 55Fe by these cells. The 55Fe taken up by the poisoned cells was found in the membrane fraction after cells were broken; 70% of the radioactivity was distributed in the outer membrane, and 30% was in the inner membrane. Isolated outer membranes from iron-starved cells were as capable of iron uptake from citrate as intact cells were. As with whole cells, [14C]citrate was not taken up by isolated outer membranes. A polyacrylamide gel electrophoresis analysis of the proteins from citrate-dialyzed outer membranes after the uptake of 55Fe revealed that the radioactivity was associated with a major band of 36,500 molecular weight.

MeSH Terms
Bacterial Proteins/metabolism Biological Transport Carrier Proteins/metabolism Cell Membrane/metabolism Cell-Free System Citrates/metabolism Iron/metabolism Iron-Binding Proteins Membrane Proteins/metabolism Molecular Weight Neisseria meningitidis/metabolism Transferrin-Binding Proteins
Chemicals
Bacterial Proteins Carrier Proteins Citrates Iron-Binding Proteins Membrane Proteins Transferrin-Binding Proteins Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Simonson C
Trivett T
DeVoe I W
References (29)
29 references, click to expand
  1. The kinetics and mechanism of iron (3) exchange between chelates and transferrin. I. The complexes of citrate and nitrilotriacetic acid.
    J Biol Chem. 1967 Jun 25;242(12):2810-5 PMID: 4961518
  2. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  3. [Metabolic products of microorganisms. 94. Comparison of sideramines as growth factors and as antagonists of the sideromycins].
    Arch Mikrobiol. 1972;82(1):55-65 PMID: 4623589
  4. Iron uptake in Salmonella typhimurium: utilization of exogenous siderochromes as iron carriers.
    J Bacteriol. 1972 Sep;111(3):731-8 PMID: 4559824
  5. The biological activity of some siderochrome derivatives.
    Biochem Biophys Res Commun. 1973 Feb 5;50(3):670-5 PMID: 4631899
  6. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.
    J Bacteriol. 1973 Sep;115(3):717-22 PMID: 4580564
  7. The inducible citrate-dependent iron transport system in Escherichia coli K12.
    Biochim Biophys Acta. 1973 Nov 30;330(1):90-101 PMID: 4587079
  8. A search for transmissible pathogenic characters in invasive strains of Escherichia coli: the discovery of a plasmid-controlled toxin and a plasmid-controlled lethal character closely associated, or identical, with colicine V.
    J Gen Microbiol. 1974 Jul;83(0):95-111 PMID: 4606141
  9. The involvement of lactoferrin in the hyposideremia of acute inflammation.
    J Exp Med. 1974 Oct 1;140(4):1068-84 PMID: 4214890
  10. Egestion of degraded meningococci by polymorphonuclear leukocytes.
    J Bacteriol. 1976 Jan;125(1):258-66 PMID: 812861
  11. Further observations on the association of the colicine V plasmid of Escherichia coli with pathogenicity and with survival in the alimentary tract.
    J Gen Microbiol. 1976 Feb;92(2):335-50 PMID: 768410
  12. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
    FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
  13. Effect of iron of the relative abundance of two large polypeptides of the Escherichia coli outer membrane.
    Biochem Biophys Res Commun. 1976 May 3;70(1):315-22 PMID: 776187
  14. Iron as a replacement for mucin in the establishment of meningococcal infection in mice.
    Can J Microbiol. 1976 Jun;22(6):832-8 PMID: 819118
  15. Genetic and physiological regulation of intrinsic proteins of the outer membrane of Salmonella typhimurium.
    J Bacteriol. 1976 Jul;127(1):498-504 PMID: 776938
  16. Increased production of the outer membrane receptors for colicins B, D and M by Escherichia coli under iron starvation.
    Biochem Biophys Res Commun. 1976 Jun 7;70(3):846-53 PMID: 7254
  17. Iron transport of Escherichia coli K-12: involvement of the colicin B receptor and of a citrate-inducible protein.
    J Bacteriol. 1976 Sep;127(3):1370-5 PMID: 783142
  18. Functional organization of the outer membrane of escherichia coli: phage and colicin receptors as components of iron uptake systems.
    J Supramol Struct. 1976;5(1):37-58 PMID: 136550
  19. Involvement of outer membrane proteins in enterochelin-mediated iron uptake in Escherichia coli.
    J Biochem. 1977 Mar;81(3):749-56 PMID: 140867
  20. Ferric enterobactin transport system in Escherichia coli K-12. Extraction, assay, and specificity of the outer membrane receptor.
    Biochemistry. 1978 May 16;17(10):1922-8 PMID: 148905
  21. Piliation and colonial morphology among laboratory strains of meningococci.
    J Clin Microbiol. 1978 Apr;7(4):379-84 PMID: 99453
  22. Iron in Neisseria meningitidis: minimum requirements, effects of limitation, and characteristics of uptake.
    J Bacteriol. 1978 Oct;136(1):35-48 PMID: 101516
  23. Neisseria meningitidis infection in mice: influence of iron, variations in virulence among strains, and pathology.
    Infect Immun. 1979 May;24(2):545-51 PMID: 110703
  24. Iron and infection.
    Microbiol Rev. 1978 Mar;42(1):45-66 PMID: 379572
  25. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli.
    Infect Immun. 1979 Dec;26(3):925-32 PMID: 160892
  26. Iron acquisition by Neisseria meningitidis in vitro.
    Infect Immun. 1980 Feb;27(2):322-34 PMID: 6445876
  27. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  28. Some aspects of microbial iron metabolism.
    Bacteriol Rev. 1957 Jun;21(2):101-11 PMID: 13436357
  29. INVESTIGATIONS ON THE ACTION OF THE IRON-CONTAINING GROWTH FACTORS, SIDERAMINES; AND IRON-CONTAINING ANTIBIOTICS, SIDEROMYCINS.
    J Gen Microbiol. 1963 Jul;32:117-21 PMID: 14045511
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.31.2.547-553.1981
Published
1981-02-00
Pages
547-53
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC351342
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.31.2.547-553.1981 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2