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PMID: 1696939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal antibodies against the 70-kilodalton iron-regulated protein of Neisseria meningitidis are bactericidal and strain specific.

Infection and immunity ·Vol. 58 ·No. 9 ·1990-09-00 ·Pages 3036-41

Pettersson A, Kuipers B, Pelzer M, Verhagen E, Tiesjema RH, Tommassen J, Poolman JT

Abstract

When grown under iron limitation, Neisseria meningitidis expresses a number of outer membrane proteins (OMPs), one of which is a 70-kilodalton (kDa) major OMP. After immunization of mice with outer membrane preparations of iron-depleted cells of strain H44/76 (B:15:P1.7,16), hybridoma cell lines producing monoclonal antibodies against the 70-kDa OMP were obtained. Some of these monoclonal antibodies demonstrated strong bactericidal activity against the homologous strain H44/76 in the presence of human complement, suggesting potential application of the 70-kDa OMP as a vaccine component. However, none of the 10 selected monoclonal antibodies was able to recognize the corresponding protein from five heterologous strains of various serosubtyping characteristics. A polyclonal anti-70-kDa OMP serum also did not react with the other strains. This result shows that immunodominant surface-exposed epitopes of the meningococcal 70-kDa iron-limitation-inducible OMP are strain specific.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigenic Variation Bacterial Outer Membrane Proteins/immunology Bacterial Proteins Bacterial Vaccines/immunology Binding, Competitive Cell Line Epitopes/immunology Iron/metabolism Iron-Binding Proteins Mice Mice, Inbred BALB C Molecular Weight Neisseria meningitidis/growth & development,immunology Periplasmic Binding Proteins
Chemicals
Antibodies, Monoclonal Bacterial Outer Membrane Proteins Bacterial Proteins Bacterial Vaccines Epitopes Iron-Binding Proteins Periplasmic Binding Proteins Iron
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pettersson A
Laboratory of Bacterial Vaccines, National Institute for Public Health and Environmental Protection, Bilthoven, The Netherlands.
Kuipers B
Pelzer M
Verhagen E
Tiesjema R H
Tommassen J
Poolman J T
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.9.3036-3041.1990
Published
1990-09-00
Pages
3036-41
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313607
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.58.9.3036-3041.1990 PMC Full Text View journal details
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