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PMID: 8391216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Basic fibroblast growth factor has a differential effect on MyoD conversion of cultured aortic smooth muscle cells from newborn and adult rats.

The American journal of pathology ·Vol. 143 ·No. 1 ·1993-07-00 ·Pages 269-82

van Neck JW, Medina JJ, Onnekink C, van der Ven PF, Bloemers HP, Schwartz SM

Abstract

MyoD is a master regulatory gene for myogenesis that also converts many mesoderm-derived cells into the skeletal muscle phenotype. Rat aortic smooth muscle cells do not contain MyoD homologous mRNA. However, expression of an exogenously supplied MyoD gene in aortic smooth muscle cells cultured from newborn and adult animals converts these cells to elongated myoblasts and myotubes expressing the skeletal muscle genes for titin, nebulin, myosin, and skeletal alpha-actin. The presence of basic fibroblast growth factor during growth and serum starvation completely inhibits MyoD-mediated conversion in cultures of newborn smooth muscle cells. However, in smooth muscle cell cultures derived from adult rats the presence of fibroblast growth factor increases the conversion frequency. The differential response of exogenous MyoD suggests that the two morphological types of aortic smooth muscle cells, one typical for the newborn rat, the other for the adult rat, represent two distinctive states of differentiation.

MeSH Terms
Aging/genetics Animals Animals, Newborn Aorta, Thoracic Base Sequence Cell Differentiation/drug effects,genetics Connectin Desmin/analysis Fibroblast Growth Factor 2/pharmacology Intermediate Filament Proteins/analysis Male Membrane Proteins/analysis Molecular Sequence Data Muscle Proteins/analysis,genetics Muscle, Smooth, Vascular/chemistry,cytology,microbiology Muscles/cytology,drug effects,microbiology MyoD Protein Myosins/analysis Protein Kinases Rats Rats, Wistar Retroviridae Infections Sequence Homology Transcription, Genetic
Chemicals
Connectin Desmin Intermediate Filament Proteins Membrane Proteins Muscle Proteins MyoD Protein nebulin Fibroblast Growth Factor 2 Protein Kinases Myosins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
van Neck J W
Department of Biochemistry, University of Nijmegen, The Netherlands.
Medina J J
Onnekink C
van der Ven P F
Bloemers H P
Schwartz S M
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1993-07-00
Pages
269-82
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886934
Subset
IM
Grants
NHLBI NIH HHS · HL03174 · United States
External Links
PubMed source PMC Full Text View journal details
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