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PMID: 1660051 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of clonal vascular smooth muscle cell lines from spontaneously hypertensive and normotensive rat aortas.

In vitro cellular & developmental biology : journal of the Tissue Culture Association ·Vol. 27A ·No. 10 ·1991-10-00 ·Pages 791-8

Hall KL, Harding JW, Hosick HL

Abstract

Vascular smooth muscle cells were isolated from the aortas of spontaneously hypertensive rats and normotensive Wistar-Kyoto rats by use of the explant method on collagen gels. Clonal cell lines derived from these enriched populations possessed ultrastructural characteristics of vascular smooth muscle cells in culture; they grew in hill and valley configuration, immunostained with the muscle actin antibody HHF35, and failed to react with von Willebrand Factor VIII antibody. Fourteen clonal cell lines were characterized for growth and ligand binding characteristics. Large variations in growth rate and cell density at saturation were exhibited by clones of both strains. Similar variability was noted for specific binding of endothelial 1 and Sar1,Ile8-angiotensin II to their receptors, indicating considerable phenotypic heterogeneity among the clonal cell lines. Six selected clones were further characterized for angiotensin II receptor linkage to G proteins. Cells of both strains exhibited comparable affinity shifts in the presence of GTP gamma S. These clonal cell lines should be useful for a variety of analyses of the comparative biology of aortic cells. It is possible that the diversity of phenotypic traits exhibited by these clones reflects the heterogeneity of vascular smooth muscle tissue found in vivo.

MeSH Terms
Actins/analysis Angiotensin II/metabolism Animals Aorta, Thoracic/cytology,metabolism,ultrastructure Cell Division Cell Membrane/metabolism,ultrastructure Cell Survival Clone Cells Culture Techniques/methods Endothelins/metabolism Female Fluorescent Antibody Technique Kinetics Microscopy, Electron Muscle, Smooth, Vascular/cytology,metabolism,ultrastructure Radioligand Assay Rats Rats, Inbred SHR Rats, Inbred WKY Receptors, Angiotensin/metabolism Receptors, Cell Surface/metabolism Receptors, Endothelin von Willebrand Factor/analysis
Chemicals
Actins Endothelins Receptors, Angiotensin Receptors, Cell Surface Receptors, Endothelin von Willebrand Factor Angiotensin II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hall K L
Department of Veterinary Comparative Anatomy, Pharmacology, and Physiology, School of Veterinary Medicine, Pullman, Washington 99164.
Harding J W
Hosick H L
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Article Info
Journal
In vitro cellular & developmental biology : journal of the Tissue Culture Association
Abbr.
In Vitro Cell Dev Biol
ISSN
0883-8364
DOI
10.1007/BF02631245
Published
1991-10-00
Pages
791-8
Language
English
Region
United States
NLM ID
8506951
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02631245
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