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PMID: 8387996 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Amplification of the Bacillus subtilis maf gene results in arrested septum formation.

Journal of bacteriology ·Vol. 175 ·No. 10 ·1993-05-00 ·Pages 3139-45

Butler YX, Abhayawardhane Y, Stewart GC

Abstract

The Bacillus subtilis homolog of the Escherichia coli morphogene orfE (of the mre operon) has been identified. The determinant is located on the chromosome immediately upstream of the mreBCD-minCD (divIVB) operon. The Maf protein shares substantial amino acid sequence identity with the E. coli OrfE protein. Introduction of the B. subtilis maf determinant on a multicopy plasmid into B. subtilis cells results in an inhibition of septation, which leads to extensive filamentation and loss of viability in the transformed cell population. Insertional inactivation of maf indicated that this gene is not essential for cell division.

Related Genes
maf mre
MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics,growth & development,ultrastructure Bacterial Proteins/biosynthesis,genetics Cell Division/genetics Chromosome Mapping Cloning, Molecular DNA Transposable Elements Gene Amplification Genes, Bacterial/genetics Molecular Sequence Data Morphogenesis/genetics Mutagenesis, Insertional Plasmids/genetics Recombinant Proteins/biosynthesis Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins DNA Transposable Elements Maf protein, Bacillus subtilis Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Butler Y X
Department of Microbiology, Univeristy of Kansas, Lawrence 66045.
Abhayawardhane Y
Stewart G C
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37 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.10.3139-3145.1993
Published
1993-05-00
Pages
3139-45
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204636
Subset
IM
Grants
NIGMS NIH HHS · GM39770 · United States
External Links
PubMed source DOI: 10.1128/jb.175.10.3139-3145.1993 PMC Full Text View journal details
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